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- W2007808295 abstract "Neurofilaments (NFs) are the intermediate filaments in neuronal cells that along with other cytoskeletal network structures play a major role in the mechanical integrity of neuronal processes. NFs are assembled from three different subunits (NF-Low (NF-L), NF-Medium (NF-M), NF-High (NF-H)) that differ mainly in the sequence length of their unstructured C-terminal sidearms. The sidearms direct the lateral associations between filaments thus forming the NF hydrogel networks (the physiologically relevant NF protein assembly state in the axoplasm). The interfilament lateral associations are predominantly electrostatic, enabled by the polyampholytic nature of the sidearms. We examine their strength and range by varying the salinity of the in vitro buffer. Furthermore, motivated by variable in vivo subunit expression in axons versus dendrites that results in variable network packing, reassembled (in vitro) binary system NF-hydrogels have revealed the different contributions of individual subunits to interfilament interactions and to network interfilament spacings [1]. Synchrotron x-ray scattering experiments have allowed us to study the changes in the microscopic structure of NF hydrogels as a function of salt and sidearm density. At high weight ratios of NF-M and NF-H, and as a function of increasing salt concentrations, NF gels exhibit an unexpectedly abrupt transition from a weakly oriented (nearly isotropic) low filament density gel with interfilament spacing d=1000Å to a highly oriented liquid crystalline gel with high filament density and d=500Å (NF-M) and 700Å (NF-H). The tunability of the network in vitro mirrors in vivo cellular control of the NF network via subunit phosphorylation, which may transition the network from a highly oriented rigid state to one with orientational plasticity. Funded by DOE-BES-DE-FG-02-06ER46314 and NSF-DMR-1101900 [1] R. Beck, J. Deek, J.B. Jones, C.R. Safinya. Nature Materials. 9, 40 (2010)." @default.
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- W2007808295 date "2012-01-01" @default.
- W2007808295 modified "2023-09-26" @default.
- W2007808295 title "Responsive and Tunable Neurofilament Protein Hydrogel Assemblies - A Synchrotron X-Ray Scattering Study of Composition and Salt Dependent Response" @default.
- W2007808295 doi "https://doi.org/10.1016/j.bpj.2011.11.1443" @default.
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