FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2007884406> ?p ?o ?g. }

• W2007884406 endingPage "30" @default.
• W2007884406 startingPage "1" @default.
• W2007884406 abstract "We demonstrate here that stilbene estrogen (diethylstilbestrol) is converted to nuclear protein binding metabolite(s) both in vitro and in vivo. In vitro reaction of DES with nuclei from hamster liver or kidney in the presence of cumene hydroperoxide or NADPH revealed binding of [3H]DES in nuclear proteins (histones; nonhistones precipitable by 2% TCA, NH2; nonhistones soluble in 2% TCA, NH30). The binding was significantly inhibited by cytochromes P450 inhibitors. In an in vitro system [3H]DES quinone, one of the metabolites of DES, was able to bind to pure nonhistone proteins RNA polymerase and DNA polymerase. The binding of [3H]DES quinone to nonhistones RNA polymerase and DNA polymerase was inhibited by low molecular weight thiols i.e. glutathione and cysteine, or thiol modifiers, such as n-ethylmaleimide, dithionitrobenzoic acid and hydroxymercuric benzoate. DES and DES metabolites inhibited transcriptional activity. In vivo [3H]DES was able to bind to nuclear proteins of hamster liver, kidneys and testes. The level of in vivo [3H]DES binding to all three types of nuclear proteins (histones, NH2, NH30) in the kidney (target organ) was two or more fold higher than that observed in the liver or testis (nontarget organs). Four nuclear NH30 proteins (mol wts.: 56, 37, 33 and 28 kDa) were irreversibly bound to [3H]DES in vivo. The in vivo binding of [3H]DES to transcriptionally active chromatin NH30 proteins also was observed. The data reported here establish that DES was able to bind to liver or kidney nuclear proteins in vitro, which was catalyzed by nuclear enzymes when fortified with an appropriate cofactor. DES quinone may be one of the protein binding metabolites. DES and DES metabolites inhibited transcriptional activity. The level of in vivo binding of [3H] DES to nuclear proteins of kidney (target organ) was double in comparison with that observed in liver or testis (nontarget organs). In vivo modifications in the chromatin proteins may be a factor in the development of DES-induced renal carcinogenesis is not clear." @default.
• W2007884406 created "2016-06-24" @default.
• W2007884406 creator A5045452181 @default.
• W2007884406 date "1980-01-01" @default.
• W2007884406 modified "2023-10-16" @default.
• W2007884406 title "Nuclear proteins and chemical carcinogenesis" @default.
• W2007884406 cites W1423919357 @default.
• W2007884406 cites W1456663821 @default.
• W2007884406 cites W1458120524 @default.
• W2007884406 cites W1483248328 @default.
• W2007884406 cites W1497807760 @default.
• W2007884406 cites W1500383334 @default.
• W2007884406 cites W1511019847 @default.
• W2007884406 cites W1531014909 @default.
• W2007884406 cites W1542243713 @default.
• W2007884406 cites W1546212643 @default.
• W2007884406 cites W1557706683 @default.
• W2007884406 cites W1561045112 @default.
• W2007884406 cites W1564215309 @default.
• W2007884406 cites W1564341683 @default.
• W2007884406 cites W1590427190 @default.
• W2007884406 cites W1590995709 @default.
• W2007884406 cites W171000395 @default.
• W2007884406 cites W172739833 @default.
• W2007884406 cites W179853234 @default.
• W2007884406 cites W187960598 @default.
• W2007884406 cites W1967946149 @default.
• W2007884406 cites W1968021983 @default.
• W2007884406 cites W1973138814 @default.
• W2007884406 cites W1973164979 @default.
• W2007884406 cites W1973430841 @default.
• W2007884406 cites W1977731392 @default.
• W2007884406 cites W1977759832 @default.
• W2007884406 cites W1981515556 @default.
• W2007884406 cites W1983541662 @default.
• W2007884406 cites W1985209489 @default.
• W2007884406 cites W1985753454 @default.
• W2007884406 cites W1986947349 @default.
• W2007884406 cites W1993613328 @default.
• W2007884406 cites W1995930438 @default.
• W2007884406 cites W1996468683 @default.
• W2007884406 cites W1996899524 @default.
• W2007884406 cites W1996956644 @default.
• W2007884406 cites W1997270665 @default.
• W2007884406 cites W1997772889 @default.
• W2007884406 cites W1997930474 @default.
• W2007884406 cites W1999975393 @default.
• W2007884406 cites W2002071813 @default.
• W2007884406 cites W2004538271 @default.
• W2007884406 cites W2006216882 @default.
• W2007884406 cites W2006431144 @default.
• W2007884406 cites W2009527539 @default.
• W2007884406 cites W2010111506 @default.
• W2007884406 cites W2011365768 @default.
• W2007884406 cites W2012740916 @default.
• W2007884406 cites W2013910592 @default.
• W2007884406 cites W2014482510 @default.
• W2007884406 cites W2016106306 @default.
• W2007884406 cites W2017309926 @default.
• W2007884406 cites W2018036356 @default.
• W2007884406 cites W2020504096 @default.
• W2007884406 cites W2021612871 @default.
• W2007884406 cites W2023408055 @default.
• W2007884406 cites W2025904415 @default.
• W2007884406 cites W2029218621 @default.
• W2007884406 cites W2032079190 @default.
• W2007884406 cites W2032258597 @default.
• W2007884406 cites W2033021469 @default.
• W2007884406 cites W2033350752 @default.
• W2007884406 cites W2034491975 @default.
• W2007884406 cites W2034581891 @default.
• W2007884406 cites W2036103440 @default.
• W2007884406 cites W2036796703 @default.
• W2007884406 cites W2041430505 @default.
• W2007884406 cites W2042877025 @default.
• W2007884406 cites W2045445406 @default.
• W2007884406 cites W2050085237 @default.
• W2007884406 cites W2051397174 @default.
• W2007884406 cites W2056215835 @default.
• W2007884406 cites W2057887575 @default.
• W2007884406 cites W2058328098 @default.
• W2007884406 cites W2062729894 @default.
• W2007884406 cites W2063180293 @default.
• W2007884406 cites W2064147522 @default.
• W2007884406 cites W2064706180 @default.
• W2007884406 cites W2067206246 @default.
• W2007884406 cites W2068899800 @default.
• W2007884406 cites W2071315443 @default.
• W2007884406 cites W2072186614 @default.
• W2007884406 cites W2078363544 @default.
• W2007884406 cites W2080840570 @default.
• W2007884406 cites W2081492608 @default.
• W2007884406 cites W2082652622 @default.
• W2007884406 cites W2082855305 @default.
• W2007884406 cites W2083675884 @default.
• W2007884406 cites W2083855077 @default.
• W2007884406 cites W2084324725 @default.
• W2007884406 cites W2085509538 @default.

• next