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• W2007905386 abstract "ET recombination is a way to engineer DNA in Escherichia coli using homologous recombination. Here we develop the potential of ET recombination in two ways relevant to complex engineering exercises such as building gene targeting constructs. First, a targeting construct was made in a single step. Second, ET recombination was used to place two unique restriction sites at precise positions in a large genomic clone. Subsequently a complex targeting construct was created by ligation with a multifunctional cassette." @default.
• W2007905386 created "2016-06-24" @default.
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• W2007905386 date "1999-09-01" @default.
• W2007905386 modified "2023-10-18" @default.
• W2007905386 title "Simplified generation of targetting constructs using ET recombination" @default.
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• W2007905386 doi "https://doi.org/10.1093/nar/27.17.e16-i" @default.
• W2007905386 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/148577" @default.
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