FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2007908413> ?p ?o ?g. }

• W2007908413 endingPage "e72380" @default.
• W2007908413 startingPage "e72380" @default.
• W2007908413 abstract "Background The identification of mosquito vectors is typically based on morphological characteristics using morphological keys of determination, which requires entomological expertise and training. The use of protein profiling by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS), which is increasingly being used for the routine identification of bacteria, has recently emerged for arthropod identification. Methods To investigate the usefulness of MALDI-TOF-MS as a mosquito identification tool, we tested protein extracts made from mosquito legs to create a database of reference spectra. The database included a total of 129 laboratory-reared and field-caught mosquito specimens consisting of 20 species, including 4 Aedes spp., 9 Anopheles spp., 4 Culex spp., Lutzia tigripes, Orthopodomyia reunionensis and Mansonia uniformis. For the validation study, blind tests were performed with 76 specimens consisting of 1 to 4 individuals per species. A cluster analysis was carried out using the MALDI-Biotyper and some spectra from all mosquito species tested. Results Biomarker mass sets containing 22 and 43 masses have been detected from 100 specimens of the Anopheles, Aedes and Culex species. By carrying out 3 blind tests, we achieved the identification of mosquito vectors at the species level, including the differentiation of An. gambiae complex, which is possible using MALDI-TOF-MS with 1.8 as the cut-off identification score. A cluster analysis performed with all available mosquito species showed that MALDI-Biotyper can distinguish between specimens at the subspecies level, as demonstrated for An gambiae M and S, but this method cannot yet be considered a reliable tool for the phylogenetic study of mosquito species. Conclusions We confirmed that even without any specific expertise, MALDI-TOF-MS profiling of mosquito leg protein extracts can be used for the rapid identification of mosquito vectors. Therefore, MALDI-TOF-MS is an alternative, efficient and inexpensive tool that can accurately identify mosquitoes collected in the field during entomological surveys." @default.
• W2007908413 created "2016-06-24" @default.
• W2007908413 creator A5009798712 @default.
• W2007908413 creator A5011540567 @default.
• W2007908413 creator A5018987442 @default.
• W2007908413 creator A5028113912 @default.
• W2007908413 creator A5052341971 @default.
• W2007908413 creator A5054560986 @default.
• W2007908413 creator A5061281389 @default.
• W2007908413 creator A5063749499 @default.
• W2007908413 creator A5074730004 @default.
• W2007908413 creator A5081717276 @default.
• W2007908413 creator A5084291847 @default.
• W2007908413 date "2013-08-15" @default.
• W2007908413 modified "2023-10-13" @default.
• W2007908413 title "Matrix-Assisted Laser Desorption Ionization - Time of Flight Mass Spectrometry: An Emerging Tool for the Rapid Identification of Mosquito Vectors" @default.
• W2007908413 cites W1548379856 @default.
• W2007908413 cites W1598603669 @default.
• W2007908413 cites W1831100553 @default.
• W2007908413 cites W1965662879 @default.
• W2007908413 cites W1966301664 @default.
• W2007908413 cites W1968770587 @default.
• W2007908413 cites W1983813938 @default.
• W2007908413 cites W1995649556 @default.
• W2007908413 cites W2004249584 @default.
• W2007908413 cites W2004277758 @default.
• W2007908413 cites W2007958461 @default.
• W2007908413 cites W2015265942 @default.
• W2007908413 cites W2015937705 @default.
• W2007908413 cites W2019150728 @default.
• W2007908413 cites W2021290940 @default.
• W2007908413 cites W2021569789 @default.
• W2007908413 cites W2023473182 @default.
• W2007908413 cites W2024073376 @default.
• W2007908413 cites W2027243607 @default.
• W2007908413 cites W2056094024 @default.
• W2007908413 cites W2056298081 @default.
• W2007908413 cites W2066579410 @default.
• W2007908413 cites W2080565248 @default.
• W2007908413 cites W2100117600 @default.
• W2007908413 cites W2107689185 @default.
• W2007908413 cites W2108084212 @default.
• W2007908413 cites W2134261696 @default.
• W2007908413 cites W2139846512 @default.
• W2007908413 cites W2141905286 @default.
• W2007908413 cites W2145659214 @default.
• W2007908413 cites W2161676160 @default.
• W2007908413 cites W2161789457 @default.
• W2007908413 cites W2165258565 @default.
• W2007908413 cites W2166960594 @default.
• W2007908413 cites W2170143378 @default.
• W2007908413 cites W2313626451 @default.
• W2007908413 cites W4293203271 @default.
• W2007908413 doi "https://doi.org/10.1371/journal.pone.0072380" @default.
• W2007908413 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3744494" @default.
• W2007908413 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23977292" @default.
• W2007908413 hasPublicationYear "2013" @default.
• W2007908413 type Work @default.
• W2007908413 sameAs 2007908413 @default.
• W2007908413 citedByCount "100" @default.
• W2007908413 countsByYear W20079084132013 @default.
• W2007908413 countsByYear W20079084132014 @default.
• W2007908413 countsByYear W20079084132015 @default.
• W2007908413 countsByYear W20079084132016 @default.
• W2007908413 countsByYear W20079084132017 @default.
• W2007908413 countsByYear W20079084132018 @default.
• W2007908413 countsByYear W20079084132019 @default.
• W2007908413 countsByYear W20079084132020 @default.
• W2007908413 countsByYear W20079084132021 @default.
• W2007908413 countsByYear W20079084132022 @default.
• W2007908413 countsByYear W20079084132023 @default.
• W2007908413 crossrefType "journal-article" @default.
• W2007908413 hasAuthorship W2007908413A5009798712 @default.
• W2007908413 hasAuthorship W2007908413A5011540567 @default.
• W2007908413 hasAuthorship W2007908413A5018987442 @default.
• W2007908413 hasAuthorship W2007908413A5028113912 @default.
• W2007908413 hasAuthorship W2007908413A5052341971 @default.
• W2007908413 hasAuthorship W2007908413A5054560986 @default.
• W2007908413 hasAuthorship W2007908413A5061281389 @default.
• W2007908413 hasAuthorship W2007908413A5063749499 @default.
• W2007908413 hasAuthorship W2007908413A5074730004 @default.
• W2007908413 hasAuthorship W2007908413A5081717276 @default.
• W2007908413 hasAuthorship W2007908413A5084291847 @default.
• W2007908413 hasBestOaLocation W20079084131 @default.
• W2007908413 hasConcept C150394285 @default.
• W2007908413 hasConcept C162356407 @default.
• W2007908413 hasConcept C162711632 @default.
• W2007908413 hasConcept C173758957 @default.
• W2007908413 hasConcept C178790620 @default.
• W2007908413 hasConcept C185592680 @default.
• W2007908413 hasConcept C18903297 @default.
• W2007908413 hasConcept C203014093 @default.
• W2007908413 hasConcept C2776802408 @default.
• W2007908413 hasConcept C2778048844 @default.
• W2007908413 hasConcept C2779518617 @default.
• W2007908413 hasConcept C2780688631 @default.
• W2007908413 hasConcept C2781375639 @default.
• W2007908413 hasConcept C43617362 @default.

• next