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Matches in SemOpenAlex for { <https://semopenalex.org/work/W2007908539> ?p ?o ?g. }

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• W2007908539 abstract "A useful strategy directed to the isolation of a required gene with a high GC content is reported. Using a degenerate oligonucleotide probe, deduced from the amino terminus of a protein, it is possible to obtain a fragment of DNA containing its encoding gene by PCR amplification. Furthermore, the cloning of a desired gene can be accomplished in two steps by using an oligonucleotide deduced (i) from an internal sequence, (ii) from a consensus sequence, or (iii) from a DNA sequence adjacent to a disrupting element (transposon, insertion sequence, cassette). This method, which could be applied to a bacteriophage, plasmid, or cosmid genomic library, has been successfully used for cloning several genes from different biological systems." @default.
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• W2007908539 date "2000-06-01" @default.
• W2007908539 modified "2023-10-17" @default.
• W2007908539 title "From a Short Amino Acidic Sequence to the Complete Gene" @default.
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• W2007908539 doi "https://doi.org/10.1006/bbrc.2000.2709" @default.
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