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- W2007935215 abstract "Abstract Objective The increasing incidence of severe fungal infections and the difficulties encountered with the existing antifungal susceptibility testing methods led us to develop a new technique based on flow cytometry, using the fluorochrome FUN -1 ® . Materials and methods We tested the susceptibility of 14 Candida spp. strains (5 reference and 9 clinical strains) to amphotericin B and fluconazole by flow cytometry versus M27-A2 CLSI reference method. Fluorescence was measured with a FACScalibur ® flow cytometer (Becton Dickinson). Amphotericin B susceptibility was studied on the 14 strains whereas fluconazole one was only performed on the five reference strains. Results Results were obtained after 30 min incubation for amphotericin B and 6 h for fluconazole. Reproducibility and repeatability tests have been performed on C. albicans ATCC 90028 and were > 95% and > 98%, respectively. The flow cytometry method resulted in clear-cut endpoints, which abolishes the source of variability of the M27-A2 reading. The results obtained showed that the patterns of susceptibility to amphotericin B were the same with the 2 methods used for all the strains. Our preliminary study with fluconazole gave the same results with the 5 control strains. Conclusion FUN-1 ® staining seems to be a rapid alternative to conventional methods to assess susceptibility of Candida spp. clinical isolates." @default.
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- W2007935215 date "2006-09-01" @default.
- W2007935215 modified "2023-09-30" @default.
- W2007935215 title "Rapid antifungal susceptibility testing of fluconazole and amphotericin B by flow cytometry using FUN-1 ® : a preliminary study" @default.
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- W2007935215 doi "https://doi.org/10.1016/j.mycmed.2006.07.003" @default.
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