Matches in SemOpenAlex for { <https://semopenalex.org/work/W2007942743> ?p ?o ?g. }
Showing items 1 to 80 of
80
with 100 items per page.
- W2007942743 endingPage "585" @default.
- W2007942743 startingPage "575" @default.
- W2007942743 abstract "A new immunomagnetic separation process that uses protein A-coated magnetic nanoclusters (PACMAN) as the separation vehicles has been developed. The nanoclusters are produced by sonicating egg yolk phosphatidylcholine and the transmembrane Fc receptor protein-A in a buffered aqueous ferrofluid suspension. The phospholipids appear to form a coating around clusters of 5-50 magnetic nanoparticles, while some of the protein-A associate with the coated layer. The PACMAN suspension shows no flocculation for several months. When this suspension is added to a mixture of chicken (CRBC) and antibody-bound sheep red blood cells (SRBC), the nanoclusters bind to the exposed Fc domains of the antibodies attached to the target SRBCs. Exposure of this mixture to a magnetic field gradient oriented against gravity causes selective migration of the magnetized SRBCs to the high magnetic field region, thus affecting a separation. Because of the overwhelming concentration of the nanoclusters in the high field region in the proximity of the magnet poles and their virtual absence in the rest of the sample away from the poles, these regions appear visually distinct. Considering these regions as different phases, the efficiency of this technique is quantified by defining a separation factor as the concentration ratio of sheep to chicken red blood cells in the high magnetic field region divided by this ratio in the low magnetic field region. Single-stage separation factors of ∼5.0 have been obtained consistently on this system. In contrast, three control experiments where the linkage between the nanoclusters and the target cells were severed at three different locations produced negligible separation, indicating minimal non-specific binding under our experimental conditions. Using a negative selection procedure, an initial mixture containing an equal number of chicken and sheep red blood cells was concentrated to 80% CRBCs in a single stage, while in three stages the concentration of CRBCs was increased to over 99%. Potential strengths of this method are the case of PACMAN formation, the ability to have an indefinitely stable suspension of possibly biocompatible magnetic clusters before addition to the target sample, and provision of a natural lipid environment for the protein-A, thus maximizing its functionality." @default.
- W2007942743 created "2016-06-24" @default.
- W2007942743 creator A5051716291 @default.
- W2007942743 creator A5081738681 @default.
- W2007942743 date "1995-03-01" @default.
- W2007942743 modified "2023-10-16" @default.
- W2007942743 title "Cell Separation Using Protein-A-Coated Magnetic Nanoclusters" @default.
- W2007942743 doi "https://doi.org/10.1006/jcis.1995.1137" @default.
- W2007942743 hasPublicationYear "1995" @default.
- W2007942743 type Work @default.
- W2007942743 sameAs 2007942743 @default.
- W2007942743 citedByCount "76" @default.
- W2007942743 countsByYear W20079427432012 @default.
- W2007942743 countsByYear W20079427432013 @default.
- W2007942743 countsByYear W20079427432015 @default.
- W2007942743 countsByYear W20079427432016 @default.
- W2007942743 countsByYear W20079427432017 @default.
- W2007942743 countsByYear W20079427432018 @default.
- W2007942743 countsByYear W20079427432019 @default.
- W2007942743 countsByYear W20079427432020 @default.
- W2007942743 countsByYear W20079427432021 @default.
- W2007942743 countsByYear W20079427432023 @default.
- W2007942743 crossrefType "journal-article" @default.
- W2007942743 hasAuthorship W2007942743A5051716291 @default.
- W2007942743 hasAuthorship W2007942743A5081738681 @default.
- W2007942743 hasConcept C105341887 @default.
- W2007942743 hasConcept C113196181 @default.
- W2007942743 hasConcept C115260700 @default.
- W2007942743 hasConcept C121332964 @default.
- W2007942743 hasConcept C12554922 @default.
- W2007942743 hasConcept C171250308 @default.
- W2007942743 hasConcept C185592680 @default.
- W2007942743 hasConcept C191897082 @default.
- W2007942743 hasConcept C192562407 @default.
- W2007942743 hasConcept C202444582 @default.
- W2007942743 hasConcept C2778402822 @default.
- W2007942743 hasConcept C2779833257 @default.
- W2007942743 hasConcept C33923547 @default.
- W2007942743 hasConcept C43617362 @default.
- W2007942743 hasConcept C5961521 @default.
- W2007942743 hasConcept C62520636 @default.
- W2007942743 hasConcept C86803240 @default.
- W2007942743 hasConceptScore W2007942743C105341887 @default.
- W2007942743 hasConceptScore W2007942743C113196181 @default.
- W2007942743 hasConceptScore W2007942743C115260700 @default.
- W2007942743 hasConceptScore W2007942743C121332964 @default.
- W2007942743 hasConceptScore W2007942743C12554922 @default.
- W2007942743 hasConceptScore W2007942743C171250308 @default.
- W2007942743 hasConceptScore W2007942743C185592680 @default.
- W2007942743 hasConceptScore W2007942743C191897082 @default.
- W2007942743 hasConceptScore W2007942743C192562407 @default.
- W2007942743 hasConceptScore W2007942743C202444582 @default.
- W2007942743 hasConceptScore W2007942743C2778402822 @default.
- W2007942743 hasConceptScore W2007942743C2779833257 @default.
- W2007942743 hasConceptScore W2007942743C33923547 @default.
- W2007942743 hasConceptScore W2007942743C43617362 @default.
- W2007942743 hasConceptScore W2007942743C5961521 @default.
- W2007942743 hasConceptScore W2007942743C62520636 @default.
- W2007942743 hasConceptScore W2007942743C86803240 @default.
- W2007942743 hasIssue "2" @default.
- W2007942743 hasLocation W20079427431 @default.
- W2007942743 hasOpenAccess W2007942743 @default.
- W2007942743 hasPrimaryLocation W20079427431 @default.
- W2007942743 hasRelatedWork W2043283315 @default.
- W2007942743 hasRelatedWork W2048946348 @default.
- W2007942743 hasRelatedWork W2164554470 @default.
- W2007942743 hasRelatedWork W2489108034 @default.
- W2007942743 hasRelatedWork W2609323171 @default.
- W2007942743 hasRelatedWork W2949637055 @default.
- W2007942743 hasRelatedWork W2953181974 @default.
- W2007942743 hasRelatedWork W2998577358 @default.
- W2007942743 hasRelatedWork W3004805157 @default.
- W2007942743 hasRelatedWork W4319590444 @default.
- W2007942743 hasVolume "170" @default.
- W2007942743 isParatext "false" @default.
- W2007942743 isRetracted "false" @default.
- W2007942743 magId "2007942743" @default.
- W2007942743 workType "article" @default.