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- W2007954588 abstract "The likelihood for improvement in the catalytic properties of Escherichia coli alkaline phosphatase was examined using site-directed mutagenesis. Mutants were constructed by introducing sequence changes into nine preselected amino acid sites within 10 A of the catalytic residue serine 102. When highly conserved residues in the family of alkaline phosphatases were mutated, many of the resulting enzymes not only maintained activity, but also exhibited greatly improved tra,. Of –170 mutant enzymes screened, 5% (eight mutants) exhibited significant increases in specific activity. In particular, a substitution by serine of a totally invariant AsplOl resulted in a 35-fold increase of specific activity over wild-type at pH 10.0. Up to 6-fold increases the kcat/km ratio were observed." @default.
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- W2007954588 date "1991-01-01" @default.
- W2007954588 modified "2023-10-03" @default.
- W2007954588 title "Mutagenesis of conserved residues within the active site of <i>Escherichia coli</i> alkaline phosphatase yields enzymes with increased <i>k</i><sub>cat</sub>" @default.
- W2007954588 doi "https://doi.org/10.1093/protein/4.7.801" @default.
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