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• W2008015151 abstract "Pyrethroids are the major class of insecticides used for mosquito control. Excessive and improper use of insecticides, however, has resulted in pyrethroid resistance, which has become a major obstacle for mosquito control. The development of pyrethroid resistance is a complex process involving many genes, and information on post-transcription regulation of pyrethroid resistance is lacking. In this study, we extracted RNA from mosquitoes in various life stages (fourth-instar larvae, pupae, male and female adult mosquitoes) from deltamethrin-sensitive (DS) and resistant (DR) strains. Using illumina sequencing, we obtained 13760296 and 12355472 reads for DS-strains and DR-strains, respectively. We identified 100 conserved miRNAs and 42 novel miRNAs derived from 21 miRNA precursors in Culex pipiens. After normalization, we identified 28 differentially expressed miRNAs between the two strains. Additionally, we found that cpp-miR-71 was significant down regulated in female adults from the DR-strain. Based on microinjection and CDC Bottle Bioassay data, we found that cpp-miR-71 may play a contributing role in deltamethrin resistance. The present study provides the firstly large-scale characterization of miRNAs in Cu. pipiens and provides evidence of post-transcription regulation. The differentially expressed miRNAs between the two strains are expected to contribute to the development of pyrethroid resistance." @default.
• W2008015151 created "2016-06-24" @default.
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• W2008015151 date "2014-12-01" @default.
• W2008015151 modified "2023-10-13" @default.
• W2008015151 title "Identification of differentially expressed microRNAs in Culex pipiens and their potential roles in pyrethroid resistance" @default.
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• W2008015151 doi "https://doi.org/10.1016/j.ibmb.2014.10.007" @default.
• W2008015151 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4420719" @default.
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