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- W2008032961 abstract "A rapid and simple microanalysis method using TopTip C18 spin tips, which are new micropipette tip spin columns for solid-phase extraction, was developed and validated for quantitative determination of methamphetamine and amphetamine in human plasma. All the solid-phase extraction procedures, including conditioning, sample loading, washing, and elution, using the TopTip C18 spin tips, were performed by centrifugation. The analytes were extracted within 7–8 min from 0.1 mL of plasma. The methanol-rich eluate was injected directly into a hydrophilic interaction liquid chromatography/tandem mass spectrometer in positive electrospray ionization mode with selected reaction monitoring. The analytes were separated on an Imtakt Unison UK-silica column using a gradient with methanol and 10 mmol/L ammonium acetate buffer as mobile phase at a flow rate of 0.5 mL/min. The recoveries of methamphetamine and amphetamine spiked into plasma were 82–95% and the limit of quantification of methamphetamine and amphetamine were 2.5 ng/mL of plasma. The calibration curves were linear over the concentration range of 2.5–500 ng/mL of plasma for both compounds. The intra-day and inter-day relative standard deviations were less than 7.5%. This fast and simple method is expected to be very useful for quantitative determination of methamphetamine and amphetamine in human plasma samples." @default.
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- W2008032961 date "2013-11-13" @default.
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- W2008032961 title "SPIN TIP SOLID-PHASE EXTRACTION AND HILIC-MS-MS FOR QUANTITATIVE DETERMINATION OF METHAMPHETAMINE AND AMPHETAMINE IN HUMAN PLASMA" @default.
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- W2008032961 doi "https://doi.org/10.1080/10826076.2012.745145" @default.
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