Matches in SemOpenAlex for { <https://semopenalex.org/work/W2008040786> ?p ?o ?g. }
Showing items 1 to 99 of
99
with 100 items per page.
- W2008040786 endingPage "309" @default.
- W2008040786 startingPage "299" @default.
- W2008040786 abstract "Analysis of the lipopolysaccharide (LPS, endotoxin) in cell sonicates of four Danish vaccine strains of Bordetella pertussis (3803, 3825, 3843 and 3860) and of purified strain 3803 LPS in sodium dodecyl sulphate-polyacrylamide gel electrophoresis by silver staining, showed identical profiles. The LPS profile revealed a dominant, brownish LPS II band and a minor, faster-migrating, black-stained LPS I band. However, the ratio of LPS I to LPS II in the preparation of purified LPS differed slightly from the cell sonicates. Using marker LPS, the molecular weights of LPS I and LPS II were estimated at 5.4 and 6.0 kD, respectively. Seven different lots of whole cell pertussis vaccine were assayed for LPS in the Limulus Amoebocyte Lysate test and were found to contain 0.9-2.8 micrograms LPS/ml. No significant difference in the content of LPS in similar dilutions of the individual strains was observed. In addition, the distribution of free and cell-bound LPS in four pertussis vaccines was investigated. Most of the LPS was found to exist as free LPS. During several months, the course of both LPS and pertussis toxin (Pt) release in freshly killed B. pertussis preparations was followed. In the first few weeks, 35-50% of the LPS was released and after 5-6 months of storage 60-80% had been released. In contrast, less than 10% of the biologically active pertussis toxin was released during the experimental period. The possibility of producing a safer whole cell pertussis vaccine by reducing the amount of free LPS without reducing the protective value correspondingly is discussed." @default.
- W2008040786 created "2016-06-24" @default.
- W2008040786 creator A5019620262 @default.
- W2008040786 creator A5037321579 @default.
- W2008040786 creator A5078768718 @default.
- W2008040786 date "1988-01-01" @default.
- W2008040786 modified "2023-10-18" @default.
- W2008040786 title "Lipopolysaccharides in a traditional pertussis vaccine" @default.
- W2008040786 cites W1533173146 @default.
- W2008040786 cites W1542023730 @default.
- W2008040786 cites W1904037768 @default.
- W2008040786 cites W1985782125 @default.
- W2008040786 cites W2011683499 @default.
- W2008040786 cites W2019083734 @default.
- W2008040786 cites W2056176279 @default.
- W2008040786 cites W2059869528 @default.
- W2008040786 cites W2068866070 @default.
- W2008040786 cites W2093505930 @default.
- W2008040786 cites W2100837269 @default.
- W2008040786 cites W2107415611 @default.
- W2008040786 cites W2135096416 @default.
- W2008040786 cites W2142880815 @default.
- W2008040786 cites W2144566802 @default.
- W2008040786 cites W2168168741 @default.
- W2008040786 cites W2187736240 @default.
- W2008040786 cites W2327955748 @default.
- W2008040786 cites W4243967441 @default.
- W2008040786 doi "https://doi.org/10.1016/0092-1157(88)90018-2" @default.
- W2008040786 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2904442" @default.
- W2008040786 hasPublicationYear "1988" @default.
- W2008040786 type Work @default.
- W2008040786 sameAs 2008040786 @default.
- W2008040786 citedByCount "9" @default.
- W2008040786 countsByYear W20080407862013 @default.
- W2008040786 countsByYear W20080407862022 @default.
- W2008040786 crossrefType "journal-article" @default.
- W2008040786 hasAuthorship W2008040786A5019620262 @default.
- W2008040786 hasAuthorship W2008040786A5037321579 @default.
- W2008040786 hasAuthorship W2008040786A5078768718 @default.
- W2008040786 hasConcept C151730666 @default.
- W2008040786 hasConcept C153911025 @default.
- W2008040786 hasConcept C159654299 @default.
- W2008040786 hasConcept C170493617 @default.
- W2008040786 hasConcept C185592680 @default.
- W2008040786 hasConcept C203014093 @default.
- W2008040786 hasConcept C2776487282 @default.
- W2008040786 hasConcept C2777367657 @default.
- W2008040786 hasConcept C2777582894 @default.
- W2008040786 hasConcept C2778754761 @default.
- W2008040786 hasConcept C2779634000 @default.
- W2008040786 hasConcept C2780801004 @default.
- W2008040786 hasConcept C49773422 @default.
- W2008040786 hasConcept C523546767 @default.
- W2008040786 hasConcept C54355233 @default.
- W2008040786 hasConcept C55493867 @default.
- W2008040786 hasConcept C80631254 @default.
- W2008040786 hasConcept C86803240 @default.
- W2008040786 hasConcept C89423630 @default.
- W2008040786 hasConceptScore W2008040786C151730666 @default.
- W2008040786 hasConceptScore W2008040786C153911025 @default.
- W2008040786 hasConceptScore W2008040786C159654299 @default.
- W2008040786 hasConceptScore W2008040786C170493617 @default.
- W2008040786 hasConceptScore W2008040786C185592680 @default.
- W2008040786 hasConceptScore W2008040786C203014093 @default.
- W2008040786 hasConceptScore W2008040786C2776487282 @default.
- W2008040786 hasConceptScore W2008040786C2777367657 @default.
- W2008040786 hasConceptScore W2008040786C2777582894 @default.
- W2008040786 hasConceptScore W2008040786C2778754761 @default.
- W2008040786 hasConceptScore W2008040786C2779634000 @default.
- W2008040786 hasConceptScore W2008040786C2780801004 @default.
- W2008040786 hasConceptScore W2008040786C49773422 @default.
- W2008040786 hasConceptScore W2008040786C523546767 @default.
- W2008040786 hasConceptScore W2008040786C54355233 @default.
- W2008040786 hasConceptScore W2008040786C55493867 @default.
- W2008040786 hasConceptScore W2008040786C80631254 @default.
- W2008040786 hasConceptScore W2008040786C86803240 @default.
- W2008040786 hasConceptScore W2008040786C89423630 @default.
- W2008040786 hasIssue "4" @default.
- W2008040786 hasLocation W20080407861 @default.
- W2008040786 hasLocation W20080407862 @default.
- W2008040786 hasOpenAccess W2008040786 @default.
- W2008040786 hasPrimaryLocation W20080407861 @default.
- W2008040786 hasRelatedWork W1971961992 @default.
- W2008040786 hasRelatedWork W2005247807 @default.
- W2008040786 hasRelatedWork W2008040786 @default.
- W2008040786 hasRelatedWork W2115749353 @default.
- W2008040786 hasRelatedWork W2132201861 @default.
- W2008040786 hasRelatedWork W2172212210 @default.
- W2008040786 hasRelatedWork W2199349852 @default.
- W2008040786 hasRelatedWork W2389723831 @default.
- W2008040786 hasRelatedWork W3208208486 @default.
- W2008040786 hasRelatedWork W640984046 @default.
- W2008040786 hasVolume "16" @default.
- W2008040786 isParatext "false" @default.
- W2008040786 isRetracted "false" @default.
- W2008040786 magId "2008040786" @default.
- W2008040786 workType "article" @default.