FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2008049650> ?p ?o ?g. }

• W2008049650 abstract "Some cell-adapted strains of foot-and-mouth disease virus (FMDV) can utilize heparan sulfate (HS) as a receptor to facilitate viral infection in cultured cells. A number of independent sites on the capsid that might be involved in FMDV-HS interaction have been studied. However, the previously reported residues do not adequately explain HS-dependent infection of two cell-adapted PanAsia-1 strains (O/Tibet/CHA/6/99tc and O/Fujian/CHA/9/99tc) of FMDV serotype O. To identify the molecular determinant(s) for the interaction of O/Tibet/CHA/6/99tc and O/Fujian/CHA/9/99tc with HS receptor, several chimeric viruses and site-directed mutants were generated by using an infectious cDNA of a non-HS-utilizing rescued virus (Cathay topotype) as the genomic backbone. Phenotypic properties of these viruses were determined by plaque assays and virus adsorption and penetration assays in cultured cells.Only two of the rescued viruses encoding VP0 of O/Tibet/CHA/6/99tc or VP1 of O/Fujian/CHA/9/99tc formed plaques on wild-type Chinese hamster ovary (WT-CHO; HS+) cells, but not on HS-negative pgsD-677 cells. The formation of plaques by these two chimeric viruses on WT-CHO cells could be abolished by the introduction of single amino acid mutations Gln-2080 → Leu in VP2 of O/Tibet/CHA/6/99tc and Lys-1083 → Glu in VP1 of O/Fujian/CHA/9/99tc, respectively. Nonetheless, the introduced mutation Leu-2080 → Gln in VP2 of O/Fujian/CHA/9/99tc for the construction of expectant recombinant plasmid led to non-infectious progeny virus in baby hamster kidney 21 (BHK-21) cells, and the site-directed mutant encoding Glu-1083 → Lys in VP1 of O/Tibet/CHA/6/99tc did not acquire the ability to produce plaques on WT-CHO cells. Significant differences in the inhibition of the infectivity of four HS-utilizing viruses by heparin and RGD-containing peptide were observed in BHK-21 cells. Interestingly, the chimeric virus encoding VP0 of O/Fujian/CHA/9/99tc, and the site-directed mutant encoding Gln-2080 → Leu in VP2 of O/Tibet/CHA/6/99tc could bind to HS, but there was no expression of the 3A protein of these two viruses in WT-CHO cells.The results suggest that the cooperation of certain specific amino acid residues in the capsid proteins of these two cell-adapted PanAsia-1 strains is essential for viral infectivity, the heparin affinity and the capability on FMDV-HS interaction." @default.
• W2008049650 created "2016-06-24" @default.
• W2008049650 creator A5003799076 @default.
• W2008049650 creator A5006346562 @default.
• W2008049650 creator A5014992075 @default.
• W2008049650 creator A5017573249 @default.
• W2008049650 creator A5022226638 @default.
• W2008049650 creator A5042017732 @default.
• W2008049650 creator A5043030184 @default.
• W2008049650 creator A5048051240 @default.
• W2008049650 creator A5063917782 @default.
• W2008049650 creator A5068200433 @default.
• W2008049650 creator A5070589231 @default.
• W2008049650 creator A5079954977 @default.
• W2008049650 creator A5088234579 @default.
• W2008049650 creator A5088282123 @default.
• W2008049650 date "2014-07-24" @default.
• W2008049650 modified "2023-10-01" @default.
• W2008049650 title "Effects of two amino acid substitutions in the capsid proteins on the interaction of two cell-adapted PanAsia-1 strains of foot-and-mouth disease virus serotype O with heparan sulfate receptor" @default.
• W2008049650 cites W1970595234 @default.
• W2008049650 cites W1970900822 @default.
• W2008049650 cites W1978908436 @default.
• W2008049650 cites W1980893886 @default.
• W2008049650 cites W1992424343 @default.
• W2008049650 cites W2003743395 @default.
• W2008049650 cites W2004425593 @default.
• W2008049650 cites W2015642465 @default.
• W2008049650 cites W2017422728 @default.
• W2008049650 cites W2023931683 @default.
• W2008049650 cites W2030653739 @default.
• W2008049650 cites W2034296843 @default.
• W2008049650 cites W2058201868 @default.
• W2008049650 cites W2060809301 @default.
• W2008049650 cites W2063120318 @default.
• W2008049650 cites W2068554926 @default.
• W2008049650 cites W2072378172 @default.
• W2008049650 cites W2077679050 @default.
• W2008049650 cites W2080537879 @default.
• W2008049650 cites W2090893051 @default.
• W2008049650 cites W2091494782 @default.
• W2008049650 cites W2093682764 @default.
• W2008049650 cites W2097556093 @default.
• W2008049650 cites W2103738533 @default.
• W2008049650 cites W2109185045 @default.
• W2008049650 cites W2111463222 @default.
• W2008049650 cites W2113532497 @default.
• W2008049650 cites W2120784335 @default.
• W2008049650 cites W2122591011 @default.
• W2008049650 cites W2133211985 @default.
• W2008049650 cites W2145804784 @default.
• W2008049650 cites W2149064406 @default.
• W2008049650 cites W2152301430 @default.
• W2008049650 cites W2153205607 @default.
• W2008049650 cites W2155623764 @default.
• W2008049650 cites W2158104323 @default.
• W2008049650 cites W2158485167 @default.
• W2008049650 cites W2159435221 @default.
• W2008049650 cites W2162661448 @default.
• W2008049650 cites W2163808447 @default.
• W2008049650 cites W2169829977 @default.
• W2008049650 cites W2183548358 @default.
• W2008049650 cites W4251144546 @default.
• W2008049650 cites W4376596386 @default.
• W2008049650 doi "https://doi.org/10.1186/1743-422x-11-132" @default.
• W2008049650 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4118260" @default.
• W2008049650 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25056022" @default.
• W2008049650 hasPublicationYear "2014" @default.
• W2008049650 type Work @default.
• W2008049650 sameAs 2008049650 @default.
• W2008049650 citedByCount "14" @default.
• W2008049650 countsByYear W20080496502018 @default.
• W2008049650 countsByYear W20080496502019 @default.
• W2008049650 countsByYear W20080496502020 @default.
• W2008049650 countsByYear W20080496502021 @default.
• W2008049650 countsByYear W20080496502022 @default.
• W2008049650 countsByYear W20080496502023 @default.
• W2008049650 crossrefType "journal-article" @default.
• W2008049650 hasAuthorship W2008049650A5003799076 @default.
• W2008049650 hasAuthorship W2008049650A5006346562 @default.
• W2008049650 hasAuthorship W2008049650A5014992075 @default.
• W2008049650 hasAuthorship W2008049650A5017573249 @default.
• W2008049650 hasAuthorship W2008049650A5022226638 @default.
• W2008049650 hasAuthorship W2008049650A5042017732 @default.
• W2008049650 hasAuthorship W2008049650A5043030184 @default.
• W2008049650 hasAuthorship W2008049650A5048051240 @default.
• W2008049650 hasAuthorship W2008049650A5063917782 @default.
• W2008049650 hasAuthorship W2008049650A5068200433 @default.
• W2008049650 hasAuthorship W2008049650A5070589231 @default.
• W2008049650 hasAuthorship W2008049650A5079954977 @default.
• W2008049650 hasAuthorship W2008049650A5088234579 @default.
• W2008049650 hasAuthorship W2008049650A5088282123 @default.
• W2008049650 hasBestOaLocation W20080496501 @default.
• W2008049650 hasConcept C10389963 @default.
• W2008049650 hasConcept C104317684 @default.
• W2008049650 hasConcept C143065580 @default.
• W2008049650 hasConcept C1491633281 @default.
• W2008049650 hasConcept C153911025 @default.
• W2008049650 hasConcept C159047783 @default.
• W2008049650 hasConcept C175656101 @default.
• W2008049650 hasConcept C202878990 @default.

• next