Matches in SemOpenAlex for { <https://semopenalex.org/work/W2008095931> ?p ?o ?g. }
Showing items 1 to 97 of
97
with 100 items per page.
- W2008095931 endingPage "67" @default.
- W2008095931 startingPage "58" @default.
- W2008095931 abstract "The anion channel protein from Clavibacter michiganense ssp. nebraskense (Schürholz, Th. et al. 1991, J. Membrane Biol. 123: 1-8) was analyzed at different concentrations of KCl and KF. At 0.8 M KCl the conductance G(V(m)) increases exponentially from 21 pS at 50 mV up to 53 pS at V(m) = 200 mV, 20 degrees C. The concentration dependence of G(V(m)) corresponds to a Michaelis-Menten type saturation function at all membrane voltage values applied (0-200 mV). The anion concentration K(0.5), where G(V(m)) has its half-maximum value, increases from 0.12 M at 50 mV to 0.24 M at 175 mV for channels in a soybean phospholipid bilayer. The voltage dependence of the single channel conductance, which is different for charged and neutral lipid bilayers, can be described either by a two-state flicker (2SF) model and the Nernst-Planck continuum theory, or by a two barrier, one-site (2B1S) model with asymmetric barriers. The increase in the number of open channels after a voltage jump from 50 mV to 150 mV has a time constant of 0.8 s. The changes of the single-channel conductance are much faster (<1 ms). The electric part of the gating process is characterized by the (reversible) molar electrical work DeltaG(theta) (el) = rhoZ(g)FV(m) approximately -1.3 RT, which corresponds to the movement of one charge of the gating charge number Z(g) = 1 across the fraction rho = DeltaV(m)/V(m) = 0.15 of the membrane voltage V(m) = 200 mV. Unlike with chloride, the single channel conductance of fluoride has a maximum at about 150 mV in the presence of the buffer PIPES (>/=5 mM, pH 6.8) with K(0.5) approximately 1 M. It is shown that the decrease in conductance is due to a blocking of the channel by the PIPES anion. In summary, the results indicate that the anion transport by the Clavibacter anion channel (CAC) does not require a voltage dependent conformation change of the CAC." @default.
- W2008095931 created "2016-06-24" @default.
- W2008095931 creator A5004929861 @default.
- W2008095931 creator A5039633285 @default.
- W2008095931 creator A5066614481 @default.
- W2008095931 date "1993-01-01" @default.
- W2008095931 modified "2023-09-30" @default.
- W2008095931 title "Single-channel analysis of the anion channel-forming protein from the plant pathogenic bacterium Clavibacter michiganense ssp. nebraskense" @default.
- W2008095931 cites W1971966801 @default.
- W2008095931 cites W1977811062 @default.
- W2008095931 cites W1986338259 @default.
- W2008095931 cites W2009269275 @default.
- W2008095931 cites W2021272552 @default.
- W2008095931 cites W2026395871 @default.
- W2008095931 cites W2047205572 @default.
- W2008095931 cites W2067241160 @default.
- W2008095931 cites W2075569960 @default.
- W2008095931 cites W2076099513 @default.
- W2008095931 cites W2078754745 @default.
- W2008095931 cites W2095883452 @default.
- W2008095931 doi "https://doi.org/10.1016/s0006-3495(93)81340-7" @default.
- W2008095931 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1262302" @default.
- W2008095931 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19431871" @default.
- W2008095931 hasPublicationYear "1993" @default.
- W2008095931 type Work @default.
- W2008095931 sameAs 2008095931 @default.
- W2008095931 citedByCount "6" @default.
- W2008095931 countsByYear W20080959312015 @default.
- W2008095931 crossrefType "journal-article" @default.
- W2008095931 hasAuthorship W2008095931A5004929861 @default.
- W2008095931 hasAuthorship W2008095931A5039633285 @default.
- W2008095931 hasAuthorship W2008095931A5066614481 @default.
- W2008095931 hasBestOaLocation W20080959311 @default.
- W2008095931 hasConcept C113196181 @default.
- W2008095931 hasConcept C121332964 @default.
- W2008095931 hasConcept C121932024 @default.
- W2008095931 hasConcept C12554922 @default.
- W2008095931 hasConcept C145148216 @default.
- W2008095931 hasConcept C170493617 @default.
- W2008095931 hasConcept C178790620 @default.
- W2008095931 hasConcept C181911157 @default.
- W2008095931 hasConcept C185592680 @default.
- W2008095931 hasConcept C192157962 @default.
- W2008095931 hasConcept C194544171 @default.
- W2008095931 hasConcept C26873012 @default.
- W2008095931 hasConcept C39944091 @default.
- W2008095931 hasConcept C41625074 @default.
- W2008095931 hasConcept C43617362 @default.
- W2008095931 hasConcept C50254741 @default.
- W2008095931 hasConcept C55493867 @default.
- W2008095931 hasConcept C8010536 @default.
- W2008095931 hasConcept C86803240 @default.
- W2008095931 hasConceptScore W2008095931C113196181 @default.
- W2008095931 hasConceptScore W2008095931C121332964 @default.
- W2008095931 hasConceptScore W2008095931C121932024 @default.
- W2008095931 hasConceptScore W2008095931C12554922 @default.
- W2008095931 hasConceptScore W2008095931C145148216 @default.
- W2008095931 hasConceptScore W2008095931C170493617 @default.
- W2008095931 hasConceptScore W2008095931C178790620 @default.
- W2008095931 hasConceptScore W2008095931C181911157 @default.
- W2008095931 hasConceptScore W2008095931C185592680 @default.
- W2008095931 hasConceptScore W2008095931C192157962 @default.
- W2008095931 hasConceptScore W2008095931C194544171 @default.
- W2008095931 hasConceptScore W2008095931C26873012 @default.
- W2008095931 hasConceptScore W2008095931C39944091 @default.
- W2008095931 hasConceptScore W2008095931C41625074 @default.
- W2008095931 hasConceptScore W2008095931C43617362 @default.
- W2008095931 hasConceptScore W2008095931C50254741 @default.
- W2008095931 hasConceptScore W2008095931C55493867 @default.
- W2008095931 hasConceptScore W2008095931C8010536 @default.
- W2008095931 hasConceptScore W2008095931C86803240 @default.
- W2008095931 hasIssue "1" @default.
- W2008095931 hasLocation W20080959311 @default.
- W2008095931 hasLocation W20080959312 @default.
- W2008095931 hasLocation W20080959313 @default.
- W2008095931 hasLocation W20080959314 @default.
- W2008095931 hasLocation W20080959315 @default.
- W2008095931 hasLocation W20080959316 @default.
- W2008095931 hasOpenAccess W2008095931 @default.
- W2008095931 hasPrimaryLocation W20080959311 @default.
- W2008095931 hasRelatedWork W1975455117 @default.
- W2008095931 hasRelatedWork W1996232825 @default.
- W2008095931 hasRelatedWork W1996488728 @default.
- W2008095931 hasRelatedWork W2016910868 @default.
- W2008095931 hasRelatedWork W2027688400 @default.
- W2008095931 hasRelatedWork W2049173849 @default.
- W2008095931 hasRelatedWork W2053312941 @default.
- W2008095931 hasRelatedWork W2060186922 @default.
- W2008095931 hasRelatedWork W2307938811 @default.
- W2008095931 hasRelatedWork W2416558953 @default.
- W2008095931 hasVolume "64" @default.
- W2008095931 isParatext "false" @default.
- W2008095931 isRetracted "false" @default.
- W2008095931 magId "2008095931" @default.
- W2008095931 workType "article" @default.