Matches in SemOpenAlex for { <https://semopenalex.org/work/W2008118081> ?p ?o ?g. }
Showing items 1 to 86 of
86
with 100 items per page.
- W2008118081 abstract "Thermus thermophilus cells harboring an expression plasmid for the aqualysin I gene secrete the mature enzyme into the medium. In an Escherichia coli expression system, a precursor of the enzyme with the C-terminal pro-sequence is accumulated in the cells, and upon treatment at 65 degrees C the active enzyme is produced. One- to 10-amino acid residue deletions, as well as complete 105-residue deletion of the C-terminal pro-sequence from the C-terminus, did not affect the production of the enzyme in E. coli cells. T. thermophilus cells harboring plasmids for mutant precursors with one- and three-residue deletions secreted the enzyme extracellularly. However, transformants harboring plasmids for mutant precursors with deletions of five or more amino acid residues could not be obtained. These results suggest that the C-terminal pro-sequence plays an important role in the extracellular secretion of the enzyme in T. thermophilus cells." @default.
- W2008118081 created "2016-06-24" @default.
- W2008118081 creator A5004580322 @default.
- W2008118081 creator A5020839989 @default.
- W2008118081 creator A5061540772 @default.
- W2008118081 creator A5090867811 @default.
- W2008118081 creator A5091456984 @default.
- W2008118081 date "1994-07-01" @default.
- W2008118081 modified "2023-10-03" @default.
- W2008118081 title "Requirement of a COOH-terminal pro-sequence for the extracellular secretion of aqualysin I (a thermophilic subtilisin-type protease) inThermus thermophilus" @default.
- W2008118081 cites W1493822833 @default.
- W2008118081 cites W1522104226 @default.
- W2008118081 cites W1541120458 @default.
- W2008118081 cites W1564407451 @default.
- W2008118081 cites W1591438776 @default.
- W2008118081 cites W1967170873 @default.
- W2008118081 cites W1968416749 @default.
- W2008118081 cites W1980283608 @default.
- W2008118081 cites W1980640496 @default.
- W2008118081 cites W2045786862 @default.
- W2008118081 cites W2063868681 @default.
- W2008118081 cites W2094275533 @default.
- W2008118081 cites W4255752213 @default.
- W2008118081 doi "https://doi.org/10.1111/j.1574-6968.1994.tb07009.x" @default.
- W2008118081 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8056296" @default.
- W2008118081 hasPublicationYear "1994" @default.
- W2008118081 type Work @default.
- W2008118081 sameAs 2008118081 @default.
- W2008118081 citedByCount "21" @default.
- W2008118081 countsByYear W20081180812013 @default.
- W2008118081 countsByYear W20081180812015 @default.
- W2008118081 crossrefType "journal-article" @default.
- W2008118081 hasAuthorship W2008118081A5004580322 @default.
- W2008118081 hasAuthorship W2008118081A5020839989 @default.
- W2008118081 hasAuthorship W2008118081A5061540772 @default.
- W2008118081 hasAuthorship W2008118081A5090867811 @default.
- W2008118081 hasAuthorship W2008118081A5091456984 @default.
- W2008118081 hasBestOaLocation W20081180811 @default.
- W2008118081 hasConcept C104317684 @default.
- W2008118081 hasConcept C143065580 @default.
- W2008118081 hasConcept C153911025 @default.
- W2008118081 hasConcept C167625842 @default.
- W2008118081 hasConcept C181199279 @default.
- W2008118081 hasConcept C22744801 @default.
- W2008118081 hasConcept C2776027139 @default.
- W2008118081 hasConcept C2779811187 @default.
- W2008118081 hasConcept C2780883830 @default.
- W2008118081 hasConcept C27911776 @default.
- W2008118081 hasConcept C28406088 @default.
- W2008118081 hasConcept C49039625 @default.
- W2008118081 hasConcept C547475151 @default.
- W2008118081 hasConcept C55493867 @default.
- W2008118081 hasConcept C86803240 @default.
- W2008118081 hasConceptScore W2008118081C104317684 @default.
- W2008118081 hasConceptScore W2008118081C143065580 @default.
- W2008118081 hasConceptScore W2008118081C153911025 @default.
- W2008118081 hasConceptScore W2008118081C167625842 @default.
- W2008118081 hasConceptScore W2008118081C181199279 @default.
- W2008118081 hasConceptScore W2008118081C22744801 @default.
- W2008118081 hasConceptScore W2008118081C2776027139 @default.
- W2008118081 hasConceptScore W2008118081C2779811187 @default.
- W2008118081 hasConceptScore W2008118081C2780883830 @default.
- W2008118081 hasConceptScore W2008118081C27911776 @default.
- W2008118081 hasConceptScore W2008118081C28406088 @default.
- W2008118081 hasConceptScore W2008118081C49039625 @default.
- W2008118081 hasConceptScore W2008118081C547475151 @default.
- W2008118081 hasConceptScore W2008118081C55493867 @default.
- W2008118081 hasConceptScore W2008118081C86803240 @default.
- W2008118081 hasLocation W20081180811 @default.
- W2008118081 hasLocation W20081180812 @default.
- W2008118081 hasOpenAccess W2008118081 @default.
- W2008118081 hasPrimaryLocation W20081180811 @default.
- W2008118081 hasRelatedWork W1606107581 @default.
- W2008118081 hasRelatedWork W1659195795 @default.
- W2008118081 hasRelatedWork W1753222786 @default.
- W2008118081 hasRelatedWork W1863230785 @default.
- W2008118081 hasRelatedWork W2028680712 @default.
- W2008118081 hasRelatedWork W2048658178 @default.
- W2008118081 hasRelatedWork W2059863900 @default.
- W2008118081 hasRelatedWork W2163191021 @default.
- W2008118081 hasRelatedWork W2181573007 @default.
- W2008118081 hasRelatedWork W2347804804 @default.
- W2008118081 isParatext "false" @default.
- W2008118081 isRetracted "false" @default.
- W2008118081 magId "2008118081" @default.
- W2008118081 workType "article" @default.