FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2008134622> ?p ?o ?g. }

• W2008134622 abstract "The transposon piggyBac is being used increasingly for genetic studies. Here, we describe modified versions of piggyBac transposase that have potentially wide-ranging applications, such as reversible transgenesis and modified targeting of insertions. piggyBac is distinguished by its ability to excise precisely, restoring the donor site to its pretransposon state. This characteristic makes piggyBac useful for reversible transgenesis, a potentially valuable feature when generating induced pluripotent stem cells without permanent alterations to genomic sequence. To avoid further genome modification following piggyBac excision by reintegration, we generated an excision competent/integration defective (Exc(+)Int(-)) transposase. Our findings also suggest the position of a target DNA-transposase interaction. Another goal of genome engineering is to develop reagents that can guide transgenes to preferred genomic regions. Others have shown that piggyBac transposase can be active when fused to a heterologous DNA-binding domain. An Exc(+)Int(-) transposase, the intrinsic targeting of which is defective, might also be a useful intermediate in generating a transposase whose integration activity could be rescued and redirected by fusion to a site-specific DNA-binding domain. We show that fusion to two designed zinc finger proteins rescued the Int(-) phenotype. Successful guided transgene integration into genomic DNA would have broad applications to gene therapy and molecular genetics. Thus, an Exc(+)Int(-) transposase is a potentially useful reagent for genome engineering and provides insight into the mechanism of transposase-target DNA interaction." @default.
• W2008134622 created "2016-06-24" @default.
• W2008134622 creator A5002358451 @default.
• W2008134622 creator A5005104278 @default.
• W2008134622 creator A5005840545 @default.
• W2008134622 creator A5007007026 @default.
• W2008134622 creator A5019388295 @default.
• W2008134622 creator A5025673070 @default.
• W2008134622 creator A5028322068 @default.
• W2008134622 creator A5047280872 @default.
• W2008134622 creator A5056388947 @default.
• W2008134622 creator A5059010465 @default.
• W2008134622 creator A5060756813 @default.
• W2008134622 creator A5085725734 @default.
• W2008134622 creator A5085800107 @default.
• W2008134622 date "2013-05-30" @default.
• W2008134622 modified "2023-10-15" @default.
• W2008134622 title "<i>piggyBac</i> transposase tools for genome engineering" @default.
• W2008134622 cites W1967446284 @default.
• W2008134622 cites W1977269173 @default.
• W2008134622 cites W1984987626 @default.
• W2008134622 cites W2000456081 @default.
• W2008134622 cites W2003688733 @default.
• W2008134622 cites W2008906567 @default.
• W2008134622 cites W2009448271 @default.
• W2008134622 cites W2009869892 @default.
• W2008134622 cites W2015403852 @default.
• W2008134622 cites W2021581968 @default.
• W2008134622 cites W2028349812 @default.
• W2008134622 cites W2031905970 @default.
• W2008134622 cites W2036840727 @default.
• W2008134622 cites W2056997870 @default.
• W2008134622 cites W2074679317 @default.
• W2008134622 cites W2097173606 @default.
• W2008134622 cites W2098290078 @default.
• W2008134622 cites W2104630023 @default.
• W2008134622 cites W2105928644 @default.
• W2008134622 cites W2110192935 @default.
• W2008134622 cites W2123784922 @default.
• W2008134622 cites W2128589652 @default.
• W2008134622 cites W2139908188 @default.
• W2008134622 cites W2146503498 @default.
• W2008134622 cites W2151346512 @default.
• W2008134622 cites W2158771952 @default.
• W2008134622 cites W2158979552 @default.
• W2008134622 cites W2160824531 @default.
• W2008134622 cites W4253392273 @default.
• W2008134622 doi "https://doi.org/10.1073/pnas.1305987110" @default.
• W2008134622 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3690869" @default.
• W2008134622 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23723351" @default.
• W2008134622 hasPublicationYear "2013" @default.
• W2008134622 type Work @default.
• W2008134622 sameAs 2008134622 @default.
• W2008134622 citedByCount "174" @default.
• W2008134622 countsByYear W20081346222013 @default.
• W2008134622 countsByYear W20081346222014 @default.
• W2008134622 countsByYear W20081346222015 @default.
• W2008134622 countsByYear W20081346222016 @default.
• W2008134622 countsByYear W20081346222017 @default.
• W2008134622 countsByYear W20081346222018 @default.
• W2008134622 countsByYear W20081346222019 @default.
• W2008134622 countsByYear W20081346222020 @default.
• W2008134622 countsByYear W20081346222021 @default.
• W2008134622 countsByYear W20081346222022 @default.
• W2008134622 countsByYear W20081346222023 @default.
• W2008134622 crossrefType "journal-article" @default.
• W2008134622 hasAuthorship W2008134622A5002358451 @default.
• W2008134622 hasAuthorship W2008134622A5005104278 @default.
• W2008134622 hasAuthorship W2008134622A5005840545 @default.
• W2008134622 hasAuthorship W2008134622A5007007026 @default.
• W2008134622 hasAuthorship W2008134622A5019388295 @default.
• W2008134622 hasAuthorship W2008134622A5025673070 @default.
• W2008134622 hasAuthorship W2008134622A5028322068 @default.
• W2008134622 hasAuthorship W2008134622A5047280872 @default.
• W2008134622 hasAuthorship W2008134622A5056388947 @default.
• W2008134622 hasAuthorship W2008134622A5059010465 @default.
• W2008134622 hasAuthorship W2008134622A5060756813 @default.
• W2008134622 hasAuthorship W2008134622A5085725734 @default.
• W2008134622 hasAuthorship W2008134622A5085800107 @default.
• W2008134622 hasBestOaLocation W20081346221 @default.
• W2008134622 hasConcept C102230213 @default.
• W2008134622 hasConcept C104317684 @default.
• W2008134622 hasConcept C141231307 @default.
• W2008134622 hasConcept C144501496 @default.
• W2008134622 hasConcept C145290725 @default.
• W2008134622 hasConcept C160775987 @default.
• W2008134622 hasConcept C172510086 @default.
• W2008134622 hasConcept C4918238 @default.
• W2008134622 hasConcept C519853106 @default.
• W2008134622 hasConcept C54355233 @default.
• W2008134622 hasConcept C70721500 @default.
• W2008134622 hasConcept C73247094 @default.
• W2008134622 hasConcept C86803240 @default.
• W2008134622 hasConcept C87073359 @default.
• W2008134622 hasConceptScore W2008134622C102230213 @default.
• W2008134622 hasConceptScore W2008134622C104317684 @default.
• W2008134622 hasConceptScore W2008134622C141231307 @default.
• W2008134622 hasConceptScore W2008134622C144501496 @default.
• W2008134622 hasConceptScore W2008134622C145290725 @default.
• W2008134622 hasConceptScore W2008134622C160775987 @default.

• next