Matches in SemOpenAlex for { <https://semopenalex.org/work/W2008143290> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W2008143290 endingPage "534" @default.
- W2008143290 startingPage "521" @default.
- W2008143290 abstract "Trypsins from the pyloric ceca of two fish species, yellow tail (Seriola quinqueradiata) and brown hakeling (Physiculus japonicus) were purified by a series of chromatographic separations. Purity increased 62- and 106-fold with approximately 55 and 10% yield for yellow tail trypsin and brown hakeling trypsin, respectively. Final enzyme preparations were homogeneous in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and the molecular weights of both enzymes were estimated to be 24 kDa by SDS-PAGE. Yellow tail and brown hakeling trypsins had maximal activity at pH 8.0 for hydrolysis of Nα-p-tosyl-L-arginine methyl ester hydrochloride and was unstable at acidic pH. The optimum temperatures for yellow tail and brown hakeling trypsins were 60 and 50C, respectively. Yellow tail trypsin was stable up to 50C, whereas brown hakeling was stable up to 40C. Both trypsins were stabilized by calcium ions. The activities of both trypsins were strongly inhibited by soybean trypsin inhibitor and Nα-p-tosyl-L-lysine chloromethyl ketone hydrochloride, and were partially inhibited by ethylenediaminetetraacetic acid. The N-terminal amino acid sequences of yellow tail trypsin and brown hakeling trypsin were determined as IVGGYECTPYSQPHQVSLNS and IVGGYECPKHSQPHQVSLNS, respectively." @default.
- W2008143290 created "2016-06-24" @default.
- W2008143290 creator A5018589373 @default.
- W2008143290 creator A5021732102 @default.
- W2008143290 creator A5055414031 @default.
- W2008143290 creator A5061203388 @default.
- W2008143290 creator A5089717239 @default.
- W2008143290 date "2006-10-01" @default.
- W2008143290 modified "2023-10-13" @default.
- W2008143290 title "COMPARATIVE STUDY OF ENZYMATIC CHARACTERISTICS OF TRYPSINS FROM THE PYLORIC CECA OF YELLOW TAIL (SERIOLA QUINQUERADIATA) AND BROWN HAKELING (PHYSICULUS JAPONICUS)" @default.
- W2008143290 cites W1775749144 @default.
- W2008143290 cites W1975176230 @default.
- W2008143290 cites W2001482753 @default.
- W2008143290 cites W2002679652 @default.
- W2008143290 cites W2012708038 @default.
- W2008143290 cites W2013820310 @default.
- W2008143290 cites W2016182813 @default.
- W2008143290 cites W2018030047 @default.
- W2008143290 cites W2021514815 @default.
- W2008143290 cites W2030410563 @default.
- W2008143290 cites W2033724013 @default.
- W2008143290 cites W2049537471 @default.
- W2008143290 cites W2054553764 @default.
- W2008143290 cites W2064700436 @default.
- W2008143290 cites W2072374953 @default.
- W2008143290 cites W2093824228 @default.
- W2008143290 cites W2099705257 @default.
- W2008143290 cites W2100837269 @default.
- W2008143290 cites W2149955275 @default.
- W2008143290 cites W2151650051 @default.
- W2008143290 cites W2158374199 @default.
- W2008143290 cites W2170682387 @default.
- W2008143290 cites W2212590994 @default.
- W2008143290 cites W2325729075 @default.
- W2008143290 cites W4238119007 @default.
- W2008143290 doi "https://doi.org/10.1111/j.1745-4514.2006.00079.x" @default.
- W2008143290 hasPublicationYear "2006" @default.
- W2008143290 type Work @default.
- W2008143290 sameAs 2008143290 @default.
- W2008143290 citedByCount "27" @default.
- W2008143290 countsByYear W20081432902012 @default.
- W2008143290 countsByYear W20081432902013 @default.
- W2008143290 countsByYear W20081432902014 @default.
- W2008143290 countsByYear W20081432902018 @default.
- W2008143290 countsByYear W20081432902019 @default.
- W2008143290 countsByYear W20081432902023 @default.
- W2008143290 crossrefType "journal-article" @default.
- W2008143290 hasAuthorship W2008143290A5018589373 @default.
- W2008143290 hasAuthorship W2008143290A5021732102 @default.
- W2008143290 hasAuthorship W2008143290A5055414031 @default.
- W2008143290 hasAuthorship W2008143290A5061203388 @default.
- W2008143290 hasAuthorship W2008143290A5089717239 @default.
- W2008143290 hasBestOaLocation W20081432901 @default.
- W2008143290 hasConcept C141814087 @default.
- W2008143290 hasConcept C178790620 @default.
- W2008143290 hasConcept C181199279 @default.
- W2008143290 hasConcept C185592680 @default.
- W2008143290 hasConcept C197404232 @default.
- W2008143290 hasConcept C2778973965 @default.
- W2008143290 hasConcept C2780340462 @default.
- W2008143290 hasConcept C43617362 @default.
- W2008143290 hasConcept C55493867 @default.
- W2008143290 hasConcept C94412978 @default.
- W2008143290 hasConceptScore W2008143290C141814087 @default.
- W2008143290 hasConceptScore W2008143290C178790620 @default.
- W2008143290 hasConceptScore W2008143290C181199279 @default.
- W2008143290 hasConceptScore W2008143290C185592680 @default.
- W2008143290 hasConceptScore W2008143290C197404232 @default.
- W2008143290 hasConceptScore W2008143290C2778973965 @default.
- W2008143290 hasConceptScore W2008143290C2780340462 @default.
- W2008143290 hasConceptScore W2008143290C43617362 @default.
- W2008143290 hasConceptScore W2008143290C55493867 @default.
- W2008143290 hasConceptScore W2008143290C94412978 @default.
- W2008143290 hasIssue "5" @default.
- W2008143290 hasLocation W20081432901 @default.
- W2008143290 hasLocation W20081432902 @default.
- W2008143290 hasOpenAccess W2008143290 @default.
- W2008143290 hasPrimaryLocation W20081432901 @default.
- W2008143290 hasRelatedWork W1556972298 @default.
- W2008143290 hasRelatedWork W1970731943 @default.
- W2008143290 hasRelatedWork W2002688036 @default.
- W2008143290 hasRelatedWork W2003527528 @default.
- W2008143290 hasRelatedWork W2004766780 @default.
- W2008143290 hasRelatedWork W2021982419 @default.
- W2008143290 hasRelatedWork W2045163633 @default.
- W2008143290 hasRelatedWork W2332260186 @default.
- W2008143290 hasRelatedWork W2387373026 @default.
- W2008143290 hasRelatedWork W2562424395 @default.
- W2008143290 hasVolume "30" @default.
- W2008143290 isParatext "false" @default.
- W2008143290 isRetracted "false" @default.
- W2008143290 magId "2008143290" @default.
- W2008143290 workType "article" @default.