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- W2008162786 abstract "► We predicted and isolated members of miR165 family from Prunus persica . ► We predicted and characterized Pp-ATHB8 , a ppe-miR165 target gene in peach. ► We validated ppe-miR165 family members and its target gene Pp-ATHB8 . ► Expression of ppe-miR165 members and Pp-ATHB8 in tissues of peach revealed. ► The mode by which ppe-miR165 regulates Pp-ATHB8 was demonstrated. A study to identify and validate Prunus persica microRNAs belonging to the ppe-miR165 family was conducted using miR-RACE where the expression of these miRNAs could be detected in leaves, flower buds, flowers and fruits of P. persica by quantitative real-time PCR (qRT-PCR) with some members of this miRNA family exhibiting tissue-specific expression. In addition, Pp-ATHB8 which is one of the potential target genes for ppe-miR165 family was experimentally verified by PPM-RACE and RLM-RACE, from which the cleavage sites of the target mRNA were mapped and the expression patterns of cleaved fragments also detected, thus demonstrating the mode by which ppe-miR165 regulates the target gene Pp-ATHB8 . Furthermore, spatiotemporal expression levels of the target gene and its cleaved fragments were analyzed by qRT-PCR, where they were found to exhibit expression trends at variance from ppe-miR165, thus indicating the cleavage mode of this miRNA on its target gene. The characterization of this important miRNA and its interaction with an equally important target gene in P. persica , further deepens our understanding of the role of ppe-miR165 in P. persica and expands the knowledge of small RNA-mediated regulation in this fruit crop." @default.
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- W2008162786 date "2012-10-01" @default.
- W2008162786 modified "2023-09-23" @default.
- W2008162786 title "Characterization of the miR165 family and its target gene Pp-ATHB8 in Prunus persica" @default.
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- W2008162786 doi "https://doi.org/10.1016/j.scienta.2012.07.020" @default.
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