FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2008175329> ?p ?o ?g. }

• W2008175329 endingPage "7294" @default.
• W2008175329 startingPage "7289" @default.
• W2008175329 abstract "An affinity gel bed was prepared by reconstitution of a transmembrane protein, the human red cell glucose transporter (Glut1), followed by steric immobilization of the proteoliposomes in small and rigid gel beads by freeze-thawing. The specific interactions between the reconstituted Glut1, the transport inhibitor cytochalasin B (CB), and the transported solute D-glucose were analyzed by isocratic chromatography of CB on the Glut1-proteoliposome gel bed. Specific retardation of CB which decreased upon inclusion of the competitor D-glucose in the eluent was observed on-line. The equilibrium constants for CB and D-glucose interaction with Glut1 (Kd 1.5 x 10(-7) M and 67 mM, respectively) obtained by use of equations derived for the affinity chromatographic analysis were consistent with values obtained by others by conventional methods. Effects of liposome composition, pH, and time on the CB binding activity of Glut1 were studied. Reconstitution of a membrane protein into a lipid environment and steric immobilization of the proteoliposomes favor retention of the protein activity. Immobilized proteoliposome affinity chromatography (IPAC) is a novel, powerful method for analysis of interactions between membrane proteins and solutes." @default.
• W2008175329 created "2016-06-24" @default.
• W2008175329 creator A5003105561 @default.
• W2008175329 creator A5059103924 @default.
• W2008175329 date "1995-06-06" @default.
• W2008175329 modified "2023-10-14" @default.
• W2008175329 title "Immobilized Proteoliposome Affinity Chromatography for Quantitative Analysis of Specific Interactions between Solutes and Membrane Proteins. Interaction of Cytochalasin B and D-Glucose with the Glucose Transporter Glut1" @default.
• W2008175329 cites W1489233823 @default.
• W2008175329 cites W1492408334 @default.
• W2008175329 cites W1498716787 @default.
• W2008175329 cites W1503802325 @default.
• W2008175329 cites W1576268619 @default.
• W2008175329 cites W1965983004 @default.
• W2008175329 cites W19713662 @default.
• W2008175329 cites W1996768883 @default.
• W2008175329 cites W2001663842 @default.
• W2008175329 cites W2003539081 @default.
• W2008175329 cites W2004221057 @default.
• W2008175329 cites W2020000210 @default.
• W2008175329 cites W2021735555 @default.
• W2008175329 cites W2022325350 @default.
• W2008175329 cites W2027984318 @default.
• W2008175329 cites W2028055043 @default.
• W2008175329 cites W2030620012 @default.
• W2008175329 cites W2037320103 @default.
• W2008175329 cites W2043498565 @default.
• W2008175329 cites W2047007472 @default.
• W2008175329 cites W2050815923 @default.
• W2008175329 cites W2064775549 @default.
• W2008175329 cites W2081190394 @default.
• W2008175329 cites W2088117642 @default.
• W2008175329 cites W2088969629 @default.
• W2008175329 cites W2094696077 @default.
• W2008175329 cites W2229355556 @default.
• W2008175329 cites W3021210829 @default.
• W2008175329 doi "https://doi.org/10.1021/bi00022a001" @default.
• W2008175329 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7779771" @default.
• W2008175329 hasPublicationYear "1995" @default.
• W2008175329 type Work @default.
• W2008175329 sameAs 2008175329 @default.
• W2008175329 citedByCount "56" @default.
• W2008175329 countsByYear W20081753292012 @default.
• W2008175329 countsByYear W20081753292014 @default.
• W2008175329 countsByYear W20081753292015 @default.
• W2008175329 countsByYear W20081753292016 @default.
• W2008175329 countsByYear W20081753292017 @default.
• W2008175329 countsByYear W20081753292020 @default.
• W2008175329 countsByYear W20081753292021 @default.
• W2008175329 crossrefType "journal-article" @default.
• W2008175329 hasAuthorship W2008175329A5003105561 @default.
• W2008175329 hasAuthorship W2008175329A5059103924 @default.
• W2008175329 hasConcept C134018914 @default.
• W2008175329 hasConcept C144647389 @default.
• W2008175329 hasConcept C161573976 @default.
• W2008175329 hasConcept C179247698 @default.
• W2008175329 hasConcept C181199279 @default.
• W2008175329 hasConcept C185154212 @default.
• W2008175329 hasConcept C185592680 @default.
• W2008175329 hasConcept C202751555 @default.
• W2008175329 hasConcept C2777952866 @default.
• W2008175329 hasConcept C2779012151 @default.
• W2008175329 hasConcept C2779306644 @default.
• W2008175329 hasConcept C41625074 @default.
• W2008175329 hasConcept C43617362 @default.
• W2008175329 hasConcept C55493867 @default.
• W2008175329 hasConcept C86803240 @default.
• W2008175329 hasConceptScore W2008175329C134018914 @default.
• W2008175329 hasConceptScore W2008175329C144647389 @default.
• W2008175329 hasConceptScore W2008175329C161573976 @default.
• W2008175329 hasConceptScore W2008175329C179247698 @default.
• W2008175329 hasConceptScore W2008175329C181199279 @default.
• W2008175329 hasConceptScore W2008175329C185154212 @default.
• W2008175329 hasConceptScore W2008175329C185592680 @default.
• W2008175329 hasConceptScore W2008175329C202751555 @default.
• W2008175329 hasConceptScore W2008175329C2777952866 @default.
• W2008175329 hasConceptScore W2008175329C2779012151 @default.
• W2008175329 hasConceptScore W2008175329C2779306644 @default.
• W2008175329 hasConceptScore W2008175329C41625074 @default.
• W2008175329 hasConceptScore W2008175329C43617362 @default.
• W2008175329 hasConceptScore W2008175329C55493867 @default.
• W2008175329 hasConceptScore W2008175329C86803240 @default.
• W2008175329 hasIssue "22" @default.
• W2008175329 hasLocation W20081753291 @default.
• W2008175329 hasLocation W20081753292 @default.
• W2008175329 hasOpenAccess W2008175329 @default.
• W2008175329 hasPrimaryLocation W20081753291 @default.
• W2008175329 hasRelatedWork W146539369 @default.
• W2008175329 hasRelatedWork W181881744 @default.
• W2008175329 hasRelatedWork W1838275629 @default.
• W2008175329 hasRelatedWork W1978402359 @default.
• W2008175329 hasRelatedWork W1990446373 @default.
• W2008175329 hasRelatedWork W2006525554 @default.
• W2008175329 hasRelatedWork W2020403538 @default.
• W2008175329 hasRelatedWork W2068494651 @default.
• W2008175329 hasRelatedWork W2144693486 @default.
• W2008175329 hasRelatedWork W3049305782 @default.
• W2008175329 hasVolume "34" @default.
• W2008175329 isParatext "false" @default.

• next