Matches in SemOpenAlex for { <https://semopenalex.org/work/W2008191145> ?p ?o ?g. }
- W2008191145 endingPage "896" @default.
- W2008191145 startingPage "884" @default.
- W2008191145 abstract "We have shown previously that electrophoretically and immunologically homogeneous polyclonal IgGs from the sera of autoimmune-prone MRL mice possess DNase activity. Here we have analyzed for the first time activation of DNase antibodies (Abs) by different metal ions. Polyclonal DNase IgGs were not active in the presence of EDTA or after Abs dialysis against EDTA, but could be activated by several externally added metal (Me(2+)) ions, with the level of activity decreasing in the order Mn(2+)> or =Mg(2+)>Ca(2+)> or =Cu(2+)>Co(2+)> or =Ni(2+)> or =Zn(2+), whereas Fe(2+) did not stimulate hydrolysis of supercoiled plasmid DNA (scDNA) by the Abs. The dependencies of the initial rate on the concentration of different Me(2+) ions were generally bell-shaped, demonstrating one to four maxima at different concentrations of Me(2+) ions in the 0.1-12 mM range, depending on the particular metal ion. In the presence of all Me(2+) ions, IgGs pre-dialyzed against EDTA produced only the relaxed form of scDNA and then sequence-independent hydrolysis of relaxed DNA followed. Addition of Cu(2+), Zn(2+), or Ca(2+) inhibited the Mg(2+)-dependent hydrolysis of scDNA, while Ni(2+), Co(2+), and Mn(2+) activated this reaction. The Mn(2+)-dependent hydrolysis of scDNA was activated by Ca(2+), Ni(2+), Co(2+), and Mg(2+) ions but was inhibited by Cu(2+) and Zn(2+). After addition of the second metal ion, only in the case of Mg(2+) and Ca(2+) or Mn(2+) ions an accumulation of linear DNA (single strand breaks closely spaced in the opposite strands of DNA) was observed. Affinity chromatography on DNA-cellulose separated DNase IgGs into many subfractions with various affinities to DNA and very different levels of the relative activity (0-100%) in the presence of Mn(2+), Ca(2+), and Mg(2+) ions. In contrast to all human DNases having a single pH optimum, mouse DNase IgGs demonstrated several pronounced pH optima between 4.5 and 9.5 and these dependencies were different in the presence of Mn(2+), Ca(2+), and Mg(2+) ions. These findings demonstrate a diversity of the ability of IgG to function at different pH and to be activated by different optimal metal cofactors. Possible reasons for the diversity of polyclonal mouse abzymes are discussed." @default.
- W2008191145 created "2016-06-24" @default.
- W2008191145 creator A5036757787 @default.
- W2008191145 creator A5051076208 @default.
- W2008191145 creator A5081504559 @default.
- W2008191145 creator A5087864126 @default.
- W2008191145 date "2007-07-01" @default.
- W2008191145 modified "2023-09-25" @default.
- W2008191145 title "Activation of DNA-hydrolyzing antibodies from the sera of autoimmune-prone MRL-lpr/lpr mice by different metal ions" @default.
- W2008191145 cites W1488562032 @default.
- W2008191145 cites W1965738576 @default.
- W2008191145 cites W1966910789 @default.
- W2008191145 cites W1968216690 @default.
- W2008191145 cites W1987131443 @default.
- W2008191145 cites W1987479282 @default.
- W2008191145 cites W1990986140 @default.
- W2008191145 cites W1991212304 @default.
- W2008191145 cites W1998522331 @default.
- W2008191145 cites W2000228895 @default.
- W2008191145 cites W2001538424 @default.
- W2008191145 cites W2022191003 @default.
- W2008191145 cites W2028111124 @default.
- W2008191145 cites W2029300932 @default.
- W2008191145 cites W2049055414 @default.
- W2008191145 cites W2050939959 @default.
- W2008191145 cites W2051165942 @default.
- W2008191145 cites W2068423037 @default.
- W2008191145 cites W2069228036 @default.
- W2008191145 cites W2070899849 @default.
- W2008191145 cites W2071314849 @default.
- W2008191145 cites W2072695170 @default.
- W2008191145 cites W2083106799 @default.
- W2008191145 cites W2085710109 @default.
- W2008191145 cites W2102381467 @default.
- W2008191145 cites W2127347361 @default.
- W2008191145 cites W2160434193 @default.
- W2008191145 cites W2164685678 @default.
- W2008191145 cites W2320349763 @default.
- W2008191145 cites W4231486972 @default.
- W2008191145 doi "https://doi.org/10.1016/j.bbapap.2007.05.006" @default.
- W2008191145 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17561457" @default.
- W2008191145 hasPublicationYear "2007" @default.
- W2008191145 type Work @default.
- W2008191145 sameAs 2008191145 @default.
- W2008191145 citedByCount "29" @default.
- W2008191145 countsByYear W20081911452012 @default.
- W2008191145 countsByYear W20081911452013 @default.
- W2008191145 countsByYear W20081911452014 @default.
- W2008191145 countsByYear W20081911452015 @default.
- W2008191145 countsByYear W20081911452016 @default.
- W2008191145 countsByYear W20081911452017 @default.
- W2008191145 countsByYear W20081911452018 @default.
- W2008191145 countsByYear W20081911452020 @default.
- W2008191145 countsByYear W20081911452021 @default.
- W2008191145 crossrefType "journal-article" @default.
- W2008191145 hasAuthorship W2008191145A5036757787 @default.
- W2008191145 hasAuthorship W2008191145A5051076208 @default.
- W2008191145 hasAuthorship W2008191145A5081504559 @default.
- W2008191145 hasAuthorship W2008191145A5087864126 @default.
- W2008191145 hasConcept C13965031 @default.
- W2008191145 hasConcept C145148216 @default.
- W2008191145 hasConcept C153911025 @default.
- W2008191145 hasConcept C159654299 @default.
- W2008191145 hasConcept C178790620 @default.
- W2008191145 hasConcept C185592680 @default.
- W2008191145 hasConcept C199164860 @default.
- W2008191145 hasConcept C203014093 @default.
- W2008191145 hasConcept C544153396 @default.
- W2008191145 hasConcept C552990157 @default.
- W2008191145 hasConcept C55493867 @default.
- W2008191145 hasConcept C86803240 @default.
- W2008191145 hasConcept C94412978 @default.
- W2008191145 hasConcept C956191 @default.
- W2008191145 hasConceptScore W2008191145C13965031 @default.
- W2008191145 hasConceptScore W2008191145C145148216 @default.
- W2008191145 hasConceptScore W2008191145C153911025 @default.
- W2008191145 hasConceptScore W2008191145C159654299 @default.
- W2008191145 hasConceptScore W2008191145C178790620 @default.
- W2008191145 hasConceptScore W2008191145C185592680 @default.
- W2008191145 hasConceptScore W2008191145C199164860 @default.
- W2008191145 hasConceptScore W2008191145C203014093 @default.
- W2008191145 hasConceptScore W2008191145C544153396 @default.
- W2008191145 hasConceptScore W2008191145C552990157 @default.
- W2008191145 hasConceptScore W2008191145C55493867 @default.
- W2008191145 hasConceptScore W2008191145C86803240 @default.
- W2008191145 hasConceptScore W2008191145C94412978 @default.
- W2008191145 hasConceptScore W2008191145C956191 @default.
- W2008191145 hasIssue "7" @default.
- W2008191145 hasLocation W20081911451 @default.
- W2008191145 hasLocation W20081911452 @default.
- W2008191145 hasOpenAccess W2008191145 @default.
- W2008191145 hasPrimaryLocation W20081911451 @default.
- W2008191145 hasRelatedWork W1945349562 @default.
- W2008191145 hasRelatedWork W2013910861 @default.
- W2008191145 hasRelatedWork W2022283669 @default.
- W2008191145 hasRelatedWork W2048856138 @default.
- W2008191145 hasRelatedWork W2075446914 @default.
- W2008191145 hasRelatedWork W2187191245 @default.