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• W2008223491 abstract "The electon transfer equilibrium and kinetics between azurin grom Alcaaligenes faecalis and cytochrome c551from Pseudomonas aeruginosa have been studied. The equilibrium constant K{[Cyt(III)] · [Az(I)]}/{[Cyt(II)] · [Az(II)]} = 0.5 at 25°C is about seven times smaller than that observed between the cytochrome c551 and the azurin both from P. aeruginosa[Rosen, P. and Pecht, I. (1976) Biochemistry,15, 775–786]. Potentiometric titrations confirmed a 43-mV difference between the mid-point potentials of +266 mV and +309m V for the Alcaligenes and Pseudomonas azurins respectively. The kinetics of the reaction between Alcaligenes azurin and Pseudomonas cytochromec551 were investigataed by the temperature-jump chemical relaxation method. Only a single relaxation mode was observed throughout the range of concentrations and temperatures examined. Thus, the slow relaxation mode was observed throughout the range of concentrations and temperatures examined. Thus, the slow relaxation time observed in the reaction between. P. aeruginosa azurin and cytochrome c551is not observed with the Alcaligenes azurin. The simplest mechanism that can therefore be ascribed to the investigated system is: This scheme is similar to that proposed earlier for the reaction between P. aeruginosa azurin and cytochrome c551 but does not involve the conformational transition proposed for azurin. The specific rates for the electron transfer are still fast: 1.8×106 M−1· s−1and 3.0×106M−1· s−1respectively at 25°C." @default.
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• W2008223491 date "1981-11-01" @default.
• W2008223491 modified "2023-09-24" @default.
• W2008223491 title "Electron Transfer between Azurin from Alcaligenes faecalis and Cytochrome c551 from Pseudomonas aeruginosa" @default.
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• W2008223491 doi "https://doi.org/10.1111/j.1432-1033.1981.tb05709.x" @default.
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