FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2008251036> ?p ?o ?g. }

• W2008251036 abstract "Human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT) (EC 2.7.7.49) with a high specific activity has been purified from the overexpressing Escherichia coli strain DH5 alpha [pJS3.7]. Steady-state kinetics of DNA synthesis catalysed by RT were analysed on polyriboadenylate 20-mer of (3'-5')deoxythymidylate [poly(rA).(dT)20] and polyribouridylate 20-mer of (3'-5')-deoxyadenylate [poly(rU).(dA)20] homopolymeric template-primers. Km values of 40 and 140 nM (3'-OH ends) and kcat values of 4 and 0.14 sec-1 were determined for the two different substrates. Oligonucleotide primers (dA)20 and (dT)20 were elongated in a terminal transferase-catalysed reaction (EC 2.7.7.31) with ddATP, 3'-dATP (cordycepin), 2',3'-epoxy-ATP and arabino-ATP; and ddTTP, 3'-azido-TTP, 3'-dUTP, 3'-F-dTTP and rUTP, respectively. The resulting oligonucleotides were hybridized to their complementary templates and the inhibitory potential of these compounds towards DNA synthesis started from unchanged primers was measured. Oligonucleotides with unextendable 3'-groups were shown to act as strong inhibitors of DNA synthesis catalysed by HIV-1 RT. In particular, poly(rA).(dT)20-[ddTMP] and poly(rU).(dA)20-[3'-dAMP] were potent competitive inhibitors, displaying Ki values of about 6 and 12 nM, respectively. Also 3'-azido-, and 3'-fluoro-terminated oligonucleotides showed competitive inhibition with inhibition constants in the range of 20-35 nM. In contrast, 2',3'-epoxy-terminated (dA)21 displayed a mixed-type inhibition with a Ki value of 67 nM. Arabino-terminated (dA)21 was found to be an uncompetitive inhibitor of HIV-1 RT with an inhibition constant of 318 nM. Arabino-terminated primers did not act as strict chain terminators because they could be elongated by HIV-1 RT. This study provides information on the structure-activity relationship of modified 3'-termini of primer molecules which might be exploited as inhibitors of HIV in the future." @default.
• W2008251036 created "2016-06-24" @default.
• W2008251036 creator A5023405423 @default.
• W2008251036 creator A5023944019 @default.
• W2008251036 creator A5088847455 @default.
• W2008251036 date "1992-06-01" @default.
• W2008251036 modified "2023-09-27" @default.
• W2008251036 title "Inhibition of human immunodeficiency virus type 1 reverse transcriptase by 3′-blocked oligonucleotide primers" @default.
• W2008251036 cites W1515069870 @default.
• W2008251036 cites W1546382247 @default.
• W2008251036 cites W1547738547 @default.
• W2008251036 cites W1548955637 @default.
• W2008251036 cites W1591761111 @default.
• W2008251036 cites W1595635303 @default.
• W2008251036 cites W1767452242 @default.
• W2008251036 cites W1968873679 @default.
• W2008251036 cites W1970893465 @default.
• W2008251036 cites W1972405546 @default.
• W2008251036 cites W1973215647 @default.
• W2008251036 cites W1977282539 @default.
• W2008251036 cites W1977767806 @default.
• W2008251036 cites W1979161571 @default.
• W2008251036 cites W1983557372 @default.
• W2008251036 cites W1991670386 @default.
• W2008251036 cites W2003611716 @default.
• W2008251036 cites W2007690393 @default.
• W2008251036 cites W2011150167 @default.
• W2008251036 cites W2011153626 @default.
• W2008251036 cites W2020889498 @default.
• W2008251036 cites W2030413237 @default.
• W2008251036 cites W2046705750 @default.
• W2008251036 cites W2054979333 @default.
• W2008251036 cites W2069294342 @default.
• W2008251036 cites W2072246943 @default.
• W2008251036 cites W2092975717 @default.
• W2008251036 cites W2096389389 @default.
• W2008251036 cites W2333399282 @default.
• W2008251036 doi "https://doi.org/10.1016/0006-2952(92)90147-b" @default.
• W2008251036 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1378738" @default.
• W2008251036 hasPublicationYear "1992" @default.
• W2008251036 type Work @default.
• W2008251036 sameAs 2008251036 @default.
• W2008251036 citedByCount "6" @default.
• W2008251036 crossrefType "journal-article" @default.
• W2008251036 hasAuthorship W2008251036A5023405423 @default.
• W2008251036 hasAuthorship W2008251036A5023944019 @default.
• W2008251036 hasAuthorship W2008251036A5088847455 @default.
• W2008251036 hasConcept C104317684 @default.
• W2008251036 hasConcept C129312508 @default.
• W2008251036 hasConcept C153911025 @default.
• W2008251036 hasConcept C156719811 @default.
• W2008251036 hasConcept C181199279 @default.
• W2008251036 hasConcept C185592680 @default.
• W2008251036 hasConcept C2780066241 @default.
• W2008251036 hasConcept C41183919 @default.
• W2008251036 hasConcept C552990157 @default.
• W2008251036 hasConcept C55493867 @default.
• W2008251036 hasConcept C56856141 @default.
• W2008251036 hasConcept C64241487 @default.
• W2008251036 hasConcept C67705224 @default.
• W2008251036 hasConcept C71240020 @default.
• W2008251036 hasConcept C86803240 @default.
• W2008251036 hasConceptScore W2008251036C104317684 @default.
• W2008251036 hasConceptScore W2008251036C129312508 @default.
• W2008251036 hasConceptScore W2008251036C153911025 @default.
• W2008251036 hasConceptScore W2008251036C156719811 @default.
• W2008251036 hasConceptScore W2008251036C181199279 @default.
• W2008251036 hasConceptScore W2008251036C185592680 @default.
• W2008251036 hasConceptScore W2008251036C2780066241 @default.
• W2008251036 hasConceptScore W2008251036C41183919 @default.
• W2008251036 hasConceptScore W2008251036C552990157 @default.
• W2008251036 hasConceptScore W2008251036C55493867 @default.
• W2008251036 hasConceptScore W2008251036C56856141 @default.
• W2008251036 hasConceptScore W2008251036C64241487 @default.
• W2008251036 hasConceptScore W2008251036C67705224 @default.
• W2008251036 hasConceptScore W2008251036C71240020 @default.
• W2008251036 hasConceptScore W2008251036C86803240 @default.
• W2008251036 hasLocation W20082510361 @default.
• W2008251036 hasLocation W20082510362 @default.
• W2008251036 hasOpenAccess W2008251036 @default.
• W2008251036 hasPrimaryLocation W20082510361 @default.
• W2008251036 hasRelatedWork W1974371829 @default.
• W2008251036 hasRelatedWork W1995986402 @default.
• W2008251036 hasRelatedWork W2004396117 @default.
• W2008251036 hasRelatedWork W2008251036 @default.
• W2008251036 hasRelatedWork W2008595053 @default.
• W2008251036 hasRelatedWork W2023644431 @default.
• W2008251036 hasRelatedWork W2068316308 @default.
• W2008251036 hasRelatedWork W2073899392 @default.
• W2008251036 hasRelatedWork W2121517408 @default.
• W2008251036 hasRelatedWork W2317663545 @default.
• W2008251036 isParatext "false" @default.
• W2008251036 isRetracted "false" @default.
• W2008251036 magId "2008251036" @default.
• W2008251036 workType "article" @default.