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- W2008276770 abstract "The chromosomal δ sequences of the yeast Saccharomyces cerevisiae were employed as recombination sites to integrate the bacterial neor gene and the yeast SUC2 gene into the yeast genome. A dominate selection method employing the aminoglycoside antibiotic G418 was used. Transformation efficiencies and growth behaviors of the transformants were studied. Transformants were obtained with more than 40 integrations; the majority of insertions were tandem with a maximum of three different insertion sites utilized at one time. After 70–100 generations of growth in nonselective medium, the high copy number SUC2-neor integrants were found to be unstable; only minor instability was observed for the neor and low copy number SUC2-neor integrants. © 1996 John Wiley & Sons, Inc." @default.
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- W2008276770 date "2000-03-26" @default.
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- W2008276770 title "G418 Selection and stability of cloned genes integrated at chromosomal δ sequences of Saccharomyces cerevisiae" @default.
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- W2008276770 doi "https://doi.org/10.1002/(sici)1097-0290(19960105)49:1<45::aid-bit6>3.0.co;2-t" @default.
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