FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2008285430> ?p ?o ?g. }

• W2008285430 endingPage "106" @default.
• W2008285430 startingPage "97" @default.
• W2008285430 abstract "Immunoblot, immunofluorescence, and complement-mediated cytolytic assays revealed that two new monoclonal antibodies raised against a membrane-enriched fraction of Toxoplasma gondii tachyzoites recognize protein P22 on the surface of the parasite. Using these monoclonal antibodies to screen a cDNA expression library in λgt11, several clones expressing recombinant fusion proteins were isolated. Subsequent screening of the library with a synthetic oligonucleotide derived from the 5′ end of one of these cDNAs permitted the isolation of additional nonexpressing clones containing the entire translated sequence. Blots of parasite RNA and DNA suggested that the corresponding gene occurs as a single copy in the tachyzoite genome. The amino acid sequence deduced from the composite cDNA indicates a primary translation product with a theoretical molecular weight of 18 959. As expected for surface protein P22, the putative polypeptide contains a predicted N-terminal signal sequence and a C-terminal hydrophobic region characteristic of proteins attached to the membrane by a glycophospholipid anchor. Recombinant fusion proteins produced by the expressing clones were recognized on immunoblots by IgG antibodies in the sera of humans with acute and chronic T. gondii infection. Antibodies selected by the fusion protein reacted predominantly with a 22-kDa antigen on immunoblots of parasite lysate." @default.
• W2008285430 created "2016-06-24" @default.
• W2008285430 creator A5009890294 @default.
• W2008285430 creator A5012534523 @default.
• W2008285430 creator A5016720776 @default.
• W2008285430 creator A5048453947 @default.
• W2008285430 creator A5070625910 @default.
• W2008285430 creator A5076864861 @default.
• W2008285430 date "1990-11-01" @default.
• W2008285430 modified "2023-09-23" @default.
• W2008285430 title "Cloning, expression, and cDNA sequence of surface antigen P22 from Toxoplasma gondii" @default.
• W2008285430 cites W1492124408 @default.
• W2008285430 cites W1547600765 @default.
• W2008285430 cites W1564857046 @default.
• W2008285430 cites W1575860126 @default.
• W2008285430 cites W1577258036 @default.
• W2008285430 cites W1585151098 @default.
• W2008285430 cites W1673056682 @default.
• W2008285430 cites W1842035623 @default.
• W2008285430 cites W1964896086 @default.
• W2008285430 cites W1967719811 @default.
• W2008285430 cites W1981143096 @default.
• W2008285430 cites W1995855588 @default.
• W2008285430 cites W2030522563 @default.
• W2008285430 cites W2100837269 @default.
• W2008285430 cites W2127212175 @default.
• W2008285430 cites W2135854205 @default.
• W2008285430 cites W2138879032 @default.
• W2008285430 cites W2168847939 @default.
• W2008285430 cites W2172006456 @default.
• W2008285430 cites W2224929038 @default.
• W2008285430 doi "https://doi.org/10.1016/0166-6851(90)90134-8" @default.
• W2008285430 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2290448" @default.
• W2008285430 hasPublicationYear "1990" @default.
• W2008285430 type Work @default.
• W2008285430 sameAs 2008285430 @default.
• W2008285430 citedByCount "93" @default.
• W2008285430 countsByYear W20082854302012 @default.
• W2008285430 countsByYear W20082854302013 @default.
• W2008285430 countsByYear W20082854302014 @default.
• W2008285430 countsByYear W20082854302016 @default.
• W2008285430 countsByYear W20082854302017 @default.
• W2008285430 countsByYear W20082854302018 @default.
• W2008285430 countsByYear W20082854302019 @default.
• W2008285430 countsByYear W20082854302020 @default.
• W2008285430 countsByYear W20082854302021 @default.
• W2008285430 countsByYear W20082854302022 @default.
• W2008285430 crossrefType "journal-article" @default.
• W2008285430 hasAuthorship W2008285430A5009890294 @default.
• W2008285430 hasAuthorship W2008285430A5012534523 @default.
• W2008285430 hasAuthorship W2008285430A5016720776 @default.
• W2008285430 hasAuthorship W2008285430A5048453947 @default.
• W2008285430 hasAuthorship W2008285430A5070625910 @default.
• W2008285430 hasAuthorship W2008285430A5076864861 @default.
• W2008285430 hasConcept C104317684 @default.
• W2008285430 hasConcept C123894998 @default.
• W2008285430 hasConcept C153911025 @default.
• W2008285430 hasConcept C159654299 @default.
• W2008285430 hasConcept C167625842 @default.
• W2008285430 hasConcept C187882448 @default.
• W2008285430 hasConcept C189482833 @default.
• W2008285430 hasConcept C2777187973 @default.
• W2008285430 hasConcept C2778128430 @default.
• W2008285430 hasConcept C2778236600 @default.
• W2008285430 hasConcept C40767141 @default.
• W2008285430 hasConcept C542903549 @default.
• W2008285430 hasConcept C54355233 @default.
• W2008285430 hasConcept C55493867 @default.
• W2008285430 hasConcept C86803240 @default.
• W2008285430 hasConceptScore W2008285430C104317684 @default.
• W2008285430 hasConceptScore W2008285430C123894998 @default.
• W2008285430 hasConceptScore W2008285430C153911025 @default.
• W2008285430 hasConceptScore W2008285430C159654299 @default.
• W2008285430 hasConceptScore W2008285430C167625842 @default.
• W2008285430 hasConceptScore W2008285430C187882448 @default.
• W2008285430 hasConceptScore W2008285430C189482833 @default.
• W2008285430 hasConceptScore W2008285430C2777187973 @default.
• W2008285430 hasConceptScore W2008285430C2778128430 @default.
• W2008285430 hasConceptScore W2008285430C2778236600 @default.
• W2008285430 hasConceptScore W2008285430C40767141 @default.
• W2008285430 hasConceptScore W2008285430C542903549 @default.
• W2008285430 hasConceptScore W2008285430C54355233 @default.
• W2008285430 hasConceptScore W2008285430C55493867 @default.
• W2008285430 hasConceptScore W2008285430C86803240 @default.
• W2008285430 hasIssue "1" @default.
• W2008285430 hasLocation W20082854301 @default.
• W2008285430 hasLocation W20082854302 @default.
• W2008285430 hasOpenAccess W2008285430 @default.
• W2008285430 hasPrimaryLocation W20082854301 @default.
• W2008285430 hasRelatedWork W1858111860 @default.
• W2008285430 hasRelatedWork W1981509814 @default.
• W2008285430 hasRelatedWork W2042689205 @default.
• W2008285430 hasRelatedWork W2076893665 @default.
• W2008285430 hasRelatedWork W2083958575 @default.
• W2008285430 hasRelatedWork W2359741505 @default.
• W2008285430 hasRelatedWork W2372950376 @default.
• W2008285430 hasRelatedWork W2387252383 @default.
• W2008285430 hasRelatedWork W2429425663 @default.

• next