FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2008307907> ?p ?o ?g. }

• W2008307907 endingPage "1432" @default.
• W2008307907 startingPage "1421" @default.
• W2008307907 abstract "For retroviruses such as HIV-1 and murine leukemia virus (MLV), active receptor recruitment and trafficking occur during viral entry. However, the underlying mechanisms and cellular factors involved in the process are largely uncharacterized. The viral receptor for ecotropic MLV (eMLV), a classical model for retrovirus infection mechanisms and pathogenesis, is mouse cationic amino acid transporter 1 (mCAT-1). Growth factor receptor-bound protein 2 (GRB2) is an adaptor protein that has been shown to couple cell surface receptors, such as epidermal growth factor receptor (EGFR) and hepatocyte growth factor receptor, to intracellular signaling events. Here we examined if GRB2 could also play a role in controlling infection by retroviruses by affecting receptor function. The GRB2 RNA interference (RNAi)-mediated suppression of endogenous GRB2 resulted in a consistent and significant reduction of virus binding and membrane fusion. The binding between eMLV and cells promoted increased GRB2-mCAT-1 interactions, as detected by immunoprecipitation. Consistently, the increased colocalization of GRB2 and mCAT-1 signals was detected by confocal microscopy. This association was time dependent and paralleled the kinetics of cell-virus membrane fusion. Interestingly, unlike the canonical binding pattern seen for GRB2 and growth factor receptors, GRB2-mCAT-1 binding does not depend on the GRB2-SH2 domain-mediated recognition of tyrosine phosphorylation on the receptor. The inhibition of endogenous GRB2 led to a reduction in surface levels of mCAT-1, which was detected by immunoprecipitation and by a direct binding assay using a recombinant MLV envelope protein receptor binding domain (RBD). Consistent with this observation, the expression of a dominant negative GRB2 mutant (R86K) resulted in the sequestration of mCAT-1 from the cell surface into intracellular vesicles. Taken together, these findings suggest a novel role for GRB2 in ecotropic MLV entry and infection by facilitating mCAT-1 trafficking." @default.
• W2008307907 created "2016-06-24" @default.
• W2008307907 creator A5021574351 @default.
• W2008307907 creator A5042358991 @default.
• W2008307907 creator A5043495215 @default.
• W2008307907 creator A5054352845 @default.
• W2008307907 creator A5068283350 @default.
• W2008307907 creator A5072561157 @default.
• W2008307907 creator A5077145917 @default.
• W2008307907 date "2012-02-01" @default.
• W2008307907 modified "2023-10-16" @default.
• W2008307907 title "GRB2 Interaction with the Ecotropic Murine Leukemia Virus Receptor, mCAT-1, Controls Virus Entry and Is Stimulated by Virus Binding" @default.
• W2008307907 cites W1484259536 @default.
• W2008307907 cites W1537954453 @default.
• W2008307907 cites W1575798288 @default.
• W2008307907 cites W1773088917 @default.
• W2008307907 cites W1828243539 @default.
• W2008307907 cites W1931238275 @default.
• W2008307907 cites W1942708165 @default.
• W2008307907 cites W1964538907 @default.
• W2008307907 cites W1973154344 @default.
• W2008307907 cites W1974448932 @default.
• W2008307907 cites W1977638337 @default.
• W2008307907 cites W1979253402 @default.
• W2008307907 cites W1980602424 @default.
• W2008307907 cites W1982485185 @default.
• W2008307907 cites W1982984035 @default.
• W2008307907 cites W1987647335 @default.
• W2008307907 cites W1989855861 @default.
• W2008307907 cites W1997307715 @default.
• W2008307907 cites W1999824494 @default.
• W2008307907 cites W2001349146 @default.
• W2008307907 cites W2002987440 @default.
• W2008307907 cites W2010558905 @default.
• W2008307907 cites W2010708749 @default.
• W2008307907 cites W2011845054 @default.
• W2008307907 cites W2012219375 @default.
• W2008307907 cites W2017140385 @default.
• W2008307907 cites W2020972755 @default.
• W2008307907 cites W2022759509 @default.
• W2008307907 cites W2028272667 @default.
• W2008307907 cites W2042695353 @default.
• W2008307907 cites W2051695714 @default.
• W2008307907 cites W2053100392 @default.
• W2008307907 cites W2058925824 @default.
• W2008307907 cites W2086627334 @default.
• W2008307907 cites W2088089215 @default.
• W2008307907 cites W2094745870 @default.
• W2008307907 cites W2115261485 @default.
• W2008307907 cites W2121556358 @default.
• W2008307907 cites W2126371427 @default.
• W2008307907 cites W2133254793 @default.
• W2008307907 cites W2133396499 @default.
• W2008307907 cites W2135793835 @default.
• W2008307907 cites W2139985114 @default.
• W2008307907 cites W2148997173 @default.
• W2008307907 cites W2160777783 @default.
• W2008307907 cites W2165685835 @default.
• W2008307907 cites W2166447284 @default.
• W2008307907 cites W2182529099 @default.
• W2008307907 cites W2263242336 @default.
• W2008307907 cites W2266482114 @default.
• W2008307907 cites W2268141202 @default.
• W2008307907 cites W4249718325 @default.
• W2008307907 cites W69352699 @default.
• W2008307907 cites W79754167 @default.
• W2008307907 doi "https://doi.org/10.1128/jvi.05993-11" @default.
• W2008307907 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3264351" @default.
• W2008307907 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22090132" @default.
• W2008307907 hasPublicationYear "2012" @default.
• W2008307907 type Work @default.
• W2008307907 sameAs 2008307907 @default.
• W2008307907 citedByCount "12" @default.
• W2008307907 countsByYear W20083079072014 @default.
• W2008307907 countsByYear W20083079072015 @default.
• W2008307907 countsByYear W20083079072017 @default.
• W2008307907 countsByYear W20083079072019 @default.
• W2008307907 countsByYear W20083079072020 @default.
• W2008307907 countsByYear W20083079072021 @default.
• W2008307907 countsByYear W20083079072022 @default.
• W2008307907 crossrefType "journal-article" @default.
• W2008307907 hasAuthorship W2008307907A5021574351 @default.
• W2008307907 hasAuthorship W2008307907A5042358991 @default.
• W2008307907 hasAuthorship W2008307907A5043495215 @default.
• W2008307907 hasAuthorship W2008307907A5054352845 @default.
• W2008307907 hasAuthorship W2008307907A5068283350 @default.
• W2008307907 hasAuthorship W2008307907A5072561157 @default.
• W2008307907 hasAuthorship W2008307907A5077145917 @default.
• W2008307907 hasBestOaLocation W20083079071 @default.
• W2008307907 hasConcept C140704245 @default.
• W2008307907 hasConcept C153911025 @default.
• W2008307907 hasConcept C159047783 @default.
• W2008307907 hasConcept C159654299 @default.
• W2008307907 hasConcept C170493617 @default.
• W2008307907 hasConcept C177917778 @default.
• W2008307907 hasConcept C183786373 @default.
• W2008307907 hasConcept C203014093 @default.
• W2008307907 hasConcept C2522874641 @default.

• next