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- W2008317513 abstract "The plasmid-borne gene hph coding for hygromycin B phosphotransferase (HPH) in Escherichia coli has been identified and its nucleotide sequence determined. The hph gene is 1026 nucleotides long, coding for a protein with a predicted Mr of 39 000. The hph gene was placed in a shuttle plasmid vector, downstream from the promoter region of the cyc 1 gene of Saccharomyces cerevisiae, and an hph construction containing a single AUG in the 5' noncoding region allowed direct selection following transformation in yeast and in E. coli. Thus the hph gene can be used in cloning vectors for both pro- and eukaryotes." @default.
- W2008317513 created "2016-06-24" @default.
- W2008317513 creator A5049778246 @default.
- W2008317513 creator A5082132115 @default.
- W2008317513 date "1983-11-01" @default.
- W2008317513 modified "2023-10-16" @default.
- W2008317513 title "Plasmid-encoded hygromycin B resistance: the sequence of hygromycin B phosphotransferase gene and its expression in Escherichia coli and Saccharomyces cerevisiae" @default.
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- W2008317513 doi "https://doi.org/10.1016/0378-1119(83)90223-8" @default.
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