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- W2008332017 abstract "Abstract When decorticated grains were germinated at 14·5°, inorganic substances moved from the endosperm, mainly the aleurone layer, to the embryo. The level of Pi rose in the embryo and endosperm, and the embryo appeared to accumulate Pi against a concentration gradient. The level of organic-P declined in the endosperm, particularly in the alcurone layer. Separated aleurone layers, incubated at 25°, released only small amounts of organic or inorganic phosphate. However, when incubated with gibberellic acid (GA 3 ), a massive release of Pi occurred at the expense of organic phosphates within the tissue. This release followed a sigmoid pattern with time following a lag and was virtually complete in 6 days. Allowing the aleurone layer to dry before incubating with GA 3 reduced or abolished the lag period of Pi release and only marginally depressed the total amount ultimately freed. In contrast, α-amylase production was depressed by the longer periods of drying. The major phosphate of the aleurone was phytate ( meso -inositol hexaphosphate, IP 6 ), but traces of 1P 4 , IP 3 , IP 2 , IP 1 , Pi and unidentified phosphates were detected. During incubation with GA 3 the IP 6 content fell, and the lower esters of inositol rose slightly and then fell in a pattern indicating that the phosphate groups of each IP 6 molecule were being sequentially hydrolysed. After 6 days incubation, the tissue phosphates were reduced to a very low level. Attempts to isolate aleurone grains containing phytate were unsuccessful." @default.
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- W2008332017 date "1974-01-01" @default.
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- W2008332017 title "Phosphate mobilization in grains of Hordeum distichon" @default.
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- W2008332017 doi "https://doi.org/10.1016/s0031-9422(00)91265-2" @default.
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