Matches in SemOpenAlex for { <https://semopenalex.org/work/W2008367068> ?p ?o ?g. }
Showing items 1 to 72 of
72
with 100 items per page.
- W2008367068 abstract "OBJECTIVE: Our objective was to determine whether an increased prevalence of Chlamydia trachomatis could be detected by the polymerase chain reaction as opposed to culture in the cervices of women with acute salpingitis. STUDY DESIGN: Endocervical samples from 15 women with laparoscopy-verified acute salpingitis and 20 women seeking medical help for conditions other than pelvic pain were tested for Chlamydia trachomatis with the polymerase chain reaction. The oligonucleotide primer pairs used were specific for a 144 bp region of the major outer membrane protein that contained a single Eco RI endonuclease cleavage site. The detection of a 144 bp band that was cleaved by Eco RI to a 103 bp band denoted a Chlamydia trachomatis -positive sample. Cervical samples were cultured for Chlamydia trachomatis with the use of McCoy cells. The lymphocyte proliferative response to Chlamydia trachomatis elementary bodies was also determined. RESULTS: Nine of the 15 women (60%) with salpingitis had positive results when tested with the polymerase chain reaction for cervical Chlamydia trachomatis . Only two of these women (13%), both of whom had positive results when tested with the polymerase chain reaction, had cultures that were positive for Chlamydia trachomatis ( p Chlamydia . Those women plus two women with unexplained recurrent abortions had positive polymerase chain reaction test results for Chlamydia trachomatis . A lymphocyte proliferative response to Chlamydia trachomatis was detected in five of eight women with salpingitis, as well as three of the other four patients, all of whom had positive polymerase chain reaction test results; lymphocytes from the remaining women were unresponsive. Follow-up cervical samples were obtained 4 to 6 months after treatment from six of the patients with salpingitis who had positive polymerase chain reaction test results; at that time five had negative polymerase chain reaction test results for Chlamydia trachomatis . CONCLUSION: The polymerase chain reaction appeared to be more sensitive and more rapid than culture in detecting Chlamydia trachomatis in the cervices of women with acute salpingitis. This assay may be of value for the early diagnosis of chlamydial infections." @default.
- W2008367068 created "2016-06-24" @default.
- W2008367068 creator A5001562111 @default.
- W2008367068 creator A5018676470 @default.
- W2008367068 creator A5052629633 @default.
- W2008367068 creator A5089058245 @default.
- W2008367068 date "1994-02-01" @default.
- W2008367068 modified "2023-09-24" @default.
- W2008367068 title "Detection of Chlamydia trachomatis by the polymerase chain reaction in the cervices of women with acute salpingitis" @default.
- W2008367068 doi "https://doi.org/10.1016/0020-7292(94)90095-7" @default.
- W2008367068 hasPublicationYear "1994" @default.
- W2008367068 type Work @default.
- W2008367068 sameAs 2008367068 @default.
- W2008367068 citedByCount "0" @default.
- W2008367068 crossrefType "journal-article" @default.
- W2008367068 hasAuthorship W2008367068A5001562111 @default.
- W2008367068 hasAuthorship W2008367068A5018676470 @default.
- W2008367068 hasAuthorship W2008367068A5052629633 @default.
- W2008367068 hasAuthorship W2008367068A5089058245 @default.
- W2008367068 hasConcept C104317684 @default.
- W2008367068 hasConcept C159047783 @default.
- W2008367068 hasConcept C203014093 @default.
- W2008367068 hasConcept C2777038808 @default.
- W2008367068 hasConcept C2777391075 @default.
- W2008367068 hasConcept C2778651837 @default.
- W2008367068 hasConcept C2780122209 @default.
- W2008367068 hasConcept C2781149074 @default.
- W2008367068 hasConcept C29456083 @default.
- W2008367068 hasConcept C49105822 @default.
- W2008367068 hasConcept C55493867 @default.
- W2008367068 hasConcept C71924100 @default.
- W2008367068 hasConcept C86803240 @default.
- W2008367068 hasConceptScore W2008367068C104317684 @default.
- W2008367068 hasConceptScore W2008367068C159047783 @default.
- W2008367068 hasConceptScore W2008367068C203014093 @default.
- W2008367068 hasConceptScore W2008367068C2777038808 @default.
- W2008367068 hasConceptScore W2008367068C2777391075 @default.
- W2008367068 hasConceptScore W2008367068C2778651837 @default.
- W2008367068 hasConceptScore W2008367068C2780122209 @default.
- W2008367068 hasConceptScore W2008367068C2781149074 @default.
- W2008367068 hasConceptScore W2008367068C29456083 @default.
- W2008367068 hasConceptScore W2008367068C49105822 @default.
- W2008367068 hasConceptScore W2008367068C55493867 @default.
- W2008367068 hasConceptScore W2008367068C71924100 @default.
- W2008367068 hasConceptScore W2008367068C86803240 @default.
- W2008367068 hasLocation W20083670681 @default.
- W2008367068 hasOpenAccess W2008367068 @default.
- W2008367068 hasPrimaryLocation W20083670681 @default.
- W2008367068 hasRelatedWork W123620404 @default.
- W2008367068 hasRelatedWork W1518823514 @default.
- W2008367068 hasRelatedWork W1826856081 @default.
- W2008367068 hasRelatedWork W1965845557 @default.
- W2008367068 hasRelatedWork W1991407160 @default.
- W2008367068 hasRelatedWork W2015166991 @default.
- W2008367068 hasRelatedWork W2051105457 @default.
- W2008367068 hasRelatedWork W2066908504 @default.
- W2008367068 hasRelatedWork W2093314958 @default.
- W2008367068 hasRelatedWork W2124127788 @default.
- W2008367068 hasRelatedWork W2127961373 @default.
- W2008367068 hasRelatedWork W2153280749 @default.
- W2008367068 hasRelatedWork W2355782273 @default.
- W2008367068 hasRelatedWork W2408029175 @default.
- W2008367068 hasRelatedWork W2409538325 @default.
- W2008367068 hasRelatedWork W2412136898 @default.
- W2008367068 hasRelatedWork W2465803147 @default.
- W2008367068 hasRelatedWork W28009545 @default.
- W2008367068 hasRelatedWork W3187727230 @default.
- W2008367068 hasRelatedWork W92755900 @default.
- W2008367068 isParatext "false" @default.
- W2008367068 isRetracted "false" @default.
- W2008367068 magId "2008367068" @default.
- W2008367068 workType "article" @default.