Matches in SemOpenAlex for { <https://semopenalex.org/work/W2008367816> ?p ?o ?g. }
- W2008367816 endingPage "173" @default.
- W2008367816 startingPage "159" @default.
- W2008367816 abstract "Although islet transplantation can achieve insulin independence in patients with type 1 diabetes, sufficient number of islets derived from two or more donors is usually required to achieve normoglycemia. Activated neutrophils and neutrophil elastase (NE), which is released from these neutrophils, can directly cause injury in islet grafts. We hypothesized that inhibition of NE improves islet isolation and islet allograft survival. We tested our hypothesis by examining the effects of modified ET-Kyoto solution supplemented with sivelestat, a NE inhibitor (S-Kyoto solution), on islet yield and viability in islet isolation and the effect of intraperitoneally injected sivelestat on islet graft survival in a mouse allotransplant model. NE and proinflammatory cytokines such as tumor necrosis factor (TNF)-α and interleukin (IL)-6 increased markedly at the end of warm digestion during islet isolation and exhibited direct cytotoxic activity against the islets causing their apoptosis. The use of S-Kyoto solution significantly improved islet yield and viability. Furthermore, treatment with sivelestat resulted in significant prolongation of islet allograft survival in recipient mice. Furthermore, serum levels of IL-6 and TNF-α at 1 and 2 weeks posttransplantation were significantly higher in islet recipients than before transplantation. Our results indicated that NE released from activated neutrophils negatively affects islet survival and that its suppression both in vitro and in vivo improved islet yield and prolonged islet graft survival. The results suggest that inhibition of NE activity could be potentially useful in islet transplantation for patients with type 1 diabetes mellitus." @default.
- W2008367816 created "2016-06-24" @default.
- W2008367816 creator A5001408222 @default.
- W2008367816 creator A5013781849 @default.
- W2008367816 creator A5028284770 @default.
- W2008367816 creator A5031314925 @default.
- W2008367816 creator A5045683397 @default.
- W2008367816 creator A5053462009 @default.
- W2008367816 creator A5057798611 @default.
- W2008367816 creator A5067647817 @default.
- W2008367816 creator A5068783815 @default.
- W2008367816 creator A5069195280 @default.
- W2008367816 creator A5073472318 @default.
- W2008367816 creator A5083677685 @default.
- W2008367816 creator A5084262609 @default.
- W2008367816 date "2013-01-01" @default.
- W2008367816 modified "2023-09-25" @default.
- W2008367816 title "Significant Improvement in Islet Yield and Survival with Modified ET-Kyoto Solution: ET-Kyoto/Neutrophil Elastase Inhibitor" @default.
- W2008367816 cites W1685600544 @default.
- W2008367816 cites W1966857537 @default.
- W2008367816 cites W1970665368 @default.
- W2008367816 cites W1971194431 @default.
- W2008367816 cites W1974417451 @default.
- W2008367816 cites W1978288865 @default.
- W2008367816 cites W1980720763 @default.
- W2008367816 cites W1981367829 @default.
- W2008367816 cites W1981432917 @default.
- W2008367816 cites W1990814244 @default.
- W2008367816 cites W1995375315 @default.
- W2008367816 cites W2000051120 @default.
- W2008367816 cites W2003616931 @default.
- W2008367816 cites W2006919788 @default.
- W2008367816 cites W2014745588 @default.
- W2008367816 cites W2015115839 @default.
- W2008367816 cites W2020631966 @default.
- W2008367816 cites W2021230180 @default.
- W2008367816 cites W2022532937 @default.
- W2008367816 cites W2025909864 @default.
- W2008367816 cites W2027508389 @default.
- W2008367816 cites W2029374135 @default.
- W2008367816 cites W2034737398 @default.
- W2008367816 cites W2035171266 @default.
- W2008367816 cites W2039558665 @default.
- W2008367816 cites W2039697569 @default.
- W2008367816 cites W2045539972 @default.
- W2008367816 cites W2046236577 @default.
- W2008367816 cites W2057190776 @default.
- W2008367816 cites W2064377986 @default.
- W2008367816 cites W2065788737 @default.
- W2008367816 cites W2068652519 @default.
- W2008367816 cites W2069096201 @default.
- W2008367816 cites W2076667388 @default.
- W2008367816 cites W2077262776 @default.
- W2008367816 cites W2089564487 @default.
- W2008367816 cites W2090939610 @default.
- W2008367816 cites W2102915416 @default.
- W2008367816 cites W2105232700 @default.
- W2008367816 cites W2105754873 @default.
- W2008367816 cites W2107109383 @default.
- W2008367816 cites W2108302537 @default.
- W2008367816 cites W2128442314 @default.
- W2008367816 cites W2133186034 @default.
- W2008367816 cites W2136073712 @default.
- W2008367816 cites W2148452017 @default.
- W2008367816 cites W2149036804 @default.
- W2008367816 cites W2152189447 @default.
- W2008367816 cites W2154443639 @default.
- W2008367816 cites W2154765637 @default.
- W2008367816 cites W2158844203 @default.
- W2008367816 cites W2162654310 @default.
- W2008367816 cites W2164721196 @default.
- W2008367816 cites W2165158922 @default.
- W2008367816 cites W2168687284 @default.
- W2008367816 cites W2169335085 @default.
- W2008367816 cites W2284379072 @default.
- W2008367816 cites W2322736537 @default.
- W2008367816 cites W2328786770 @default.
- W2008367816 doi "https://doi.org/10.3727/096368912x637028" @default.
- W2008367816 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22472201" @default.
- W2008367816 hasPublicationYear "2013" @default.
- W2008367816 type Work @default.
- W2008367816 sameAs 2008367816 @default.
- W2008367816 citedByCount "6" @default.
- W2008367816 countsByYear W20083678162015 @default.
- W2008367816 countsByYear W20083678162016 @default.
- W2008367816 countsByYear W20083678162018 @default.
- W2008367816 countsByYear W20083678162022 @default.
- W2008367816 crossrefType "journal-article" @default.
- W2008367816 hasAuthorship W2008367816A5001408222 @default.
- W2008367816 hasAuthorship W2008367816A5013781849 @default.
- W2008367816 hasAuthorship W2008367816A5028284770 @default.
- W2008367816 hasAuthorship W2008367816A5031314925 @default.
- W2008367816 hasAuthorship W2008367816A5045683397 @default.
- W2008367816 hasAuthorship W2008367816A5053462009 @default.
- W2008367816 hasAuthorship W2008367816A5057798611 @default.
- W2008367816 hasAuthorship W2008367816A5067647817 @default.
- W2008367816 hasAuthorship W2008367816A5068783815 @default.
- W2008367816 hasAuthorship W2008367816A5069195280 @default.