Matches in SemOpenAlex for { <https://semopenalex.org/work/W2008372272> ?p ?o ?g. }
- W2008372272 endingPage "249" @default.
- W2008372272 startingPage "245" @default.
- W2008372272 abstract "A multiplex reverse transcriptase-polymerase chain reaction (mRT-PCR) was developed and optimized for the detection of type A influenza virus; the assay simultaneously differentiates avian H5, H7 and H9 hemagglutinin subtypes. Four sets of specific oligonucleotide primers were used in this test for type A influenza virus, H5, H7 and H9 heamagglutinin subtypes. The mRT-PCR DNA products were visualized by gel electrophoresis and consisted of fragments of 860 bp for H5, 634 bp for H7, 488 bp for H9 hemagglutinin subtypes, and 244 bp for type A influenza virus. The common set primers for type A influenza virus were able to amplify a 244 bp DNA band for any of the other subtypes of AIV. The mRT-PCR assay developed in this study was found to be sensitive and specific. Detection limit for PCR-amplified DNA products was 100 pg for the subtypes H5, H7, and H9 and 10 pg for type A influenza virus in all subtypes. No specific amplification bands of the same sizes (860, 634 and 488 bp) could be amplified for RNA of other influenza hemagglutinin subtypes, nor specific amplification bands of type A influenza (244 bp) for other viral or bacterial pathogens." @default.
- W2008372272 created "2016-06-24" @default.
- W2008372272 creator A5045443752 @default.
- W2008372272 creator A5059948762 @default.
- W2008372272 creator A5067756468 @default.
- W2008372272 creator A5076596429 @default.
- W2008372272 creator A5078466440 @default.
- W2008372272 creator A5078760161 @default.
- W2008372272 creator A5084535169 @default.
- W2008372272 date "2006-06-01" @default.
- W2008372272 modified "2023-09-24" @default.
- W2008372272 title "A multiplex RT-PCR for detection of type A influenza virus and differentiation of avian H5, H7, and H9 hemagglutinin subtypes" @default.
- W2008372272 cites W1480510611 @default.
- W2008372272 cites W1588742570 @default.
- W2008372272 cites W1677658080 @default.
- W2008372272 cites W1994766129 @default.
- W2008372272 cites W2074766065 @default.
- W2008372272 cites W2082816502 @default.
- W2008372272 cites W2094168371 @default.
- W2008372272 cites W2111323042 @default.
- W2008372272 cites W2113827628 @default.
- W2008372272 cites W2113884542 @default.
- W2008372272 cites W2122385379 @default.
- W2008372272 cites W2153608890 @default.
- W2008372272 cites W2162384187 @default.
- W2008372272 cites W2169706295 @default.
- W2008372272 cites W2170600045 @default.
- W2008372272 cites W2195941368 @default.
- W2008372272 doi "https://doi.org/10.1016/j.mcp.2006.01.003" @default.
- W2008372272 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16542820" @default.
- W2008372272 hasPublicationYear "2006" @default.
- W2008372272 type Work @default.
- W2008372272 sameAs 2008372272 @default.
- W2008372272 citedByCount "76" @default.
- W2008372272 countsByYear W20083722722012 @default.
- W2008372272 countsByYear W20083722722013 @default.
- W2008372272 countsByYear W20083722722014 @default.
- W2008372272 countsByYear W20083722722015 @default.
- W2008372272 countsByYear W20083722722016 @default.
- W2008372272 countsByYear W20083722722017 @default.
- W2008372272 countsByYear W20083722722018 @default.
- W2008372272 countsByYear W20083722722019 @default.
- W2008372272 countsByYear W20083722722020 @default.
- W2008372272 countsByYear W20083722722021 @default.
- W2008372272 countsByYear W20083722722022 @default.
- W2008372272 countsByYear W20083722722023 @default.
- W2008372272 crossrefType "journal-article" @default.
- W2008372272 hasAuthorship W2008372272A5045443752 @default.
- W2008372272 hasAuthorship W2008372272A5059948762 @default.
- W2008372272 hasAuthorship W2008372272A5067756468 @default.
- W2008372272 hasAuthorship W2008372272A5076596429 @default.
- W2008372272 hasAuthorship W2008372272A5078466440 @default.
- W2008372272 hasAuthorship W2008372272A5078760161 @default.
- W2008372272 hasAuthorship W2008372272A5084535169 @default.
- W2008372272 hasConcept C104317684 @default.
- W2008372272 hasConcept C142724271 @default.
- W2008372272 hasConcept C153911025 @default.
- W2008372272 hasConcept C159047783 @default.
- W2008372272 hasConcept C2522874641 @default.
- W2008372272 hasConcept C2777546802 @default.
- W2008372272 hasConcept C2778692840 @default.
- W2008372272 hasConcept C2779134260 @default.
- W2008372272 hasConcept C2781188995 @default.
- W2008372272 hasConcept C3008058167 @default.
- W2008372272 hasConcept C49105822 @default.
- W2008372272 hasConcept C524204448 @default.
- W2008372272 hasConcept C54355233 @default.
- W2008372272 hasConcept C71924100 @default.
- W2008372272 hasConcept C82988372 @default.
- W2008372272 hasConcept C86803240 @default.
- W2008372272 hasConcept C90583042 @default.
- W2008372272 hasConceptScore W2008372272C104317684 @default.
- W2008372272 hasConceptScore W2008372272C142724271 @default.
- W2008372272 hasConceptScore W2008372272C153911025 @default.
- W2008372272 hasConceptScore W2008372272C159047783 @default.
- W2008372272 hasConceptScore W2008372272C2522874641 @default.
- W2008372272 hasConceptScore W2008372272C2777546802 @default.
- W2008372272 hasConceptScore W2008372272C2778692840 @default.
- W2008372272 hasConceptScore W2008372272C2779134260 @default.
- W2008372272 hasConceptScore W2008372272C2781188995 @default.
- W2008372272 hasConceptScore W2008372272C3008058167 @default.
- W2008372272 hasConceptScore W2008372272C49105822 @default.
- W2008372272 hasConceptScore W2008372272C524204448 @default.
- W2008372272 hasConceptScore W2008372272C54355233 @default.
- W2008372272 hasConceptScore W2008372272C71924100 @default.
- W2008372272 hasConceptScore W2008372272C82988372 @default.
- W2008372272 hasConceptScore W2008372272C86803240 @default.
- W2008372272 hasConceptScore W2008372272C90583042 @default.
- W2008372272 hasIssue "3-4" @default.
- W2008372272 hasLocation W20083722721 @default.
- W2008372272 hasLocation W20083722722 @default.
- W2008372272 hasOpenAccess W2008372272 @default.
- W2008372272 hasPrimaryLocation W20083722721 @default.
- W2008372272 hasRelatedWork W1972800817 @default.
- W2008372272 hasRelatedWork W2049614586 @default.
- W2008372272 hasRelatedWork W2079652770 @default.
- W2008372272 hasRelatedWork W2149958759 @default.
- W2008372272 hasRelatedWork W2157882190 @default.