FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2008396783> ?p ?o ?g. }

• W2008396783 abstract "Abstract Salmonid cryptobiosis is caused by the haemoflagellate, Cryptobia (Trypanoplasma) salmositica Katz 1951. The parasite has been reported from all species of Pacific Oncorhynchus spp. on the west coast of North America. It is recognized as a lethal pathogen in semi-natural and intensive salmon culture facilities, and there is no chemotherapy against the parasite. Innate and acquired immunity have been successfully exploited to experimentally protect susceptible fish. The third approach in the protective strategy is to use monoclonal antibody technology to develop a sensitive technique for the detection of parasite antigen, and to identify a protective monoclonal antibody and its epitope. A murine IgG3 monoclonal antibody (MAb-007) against the 47 kDa polypeptide of C. salmositica was used to develop an antigen-capture ELISA diagnostic technique. The reactions were negative before infection or vaccination, but were positive 1–9 weeks after infection with the pathogen or vaccination with an attenuated strain. MAb-007 recognizes a cytoplasmic epitope, which is excreted/secreted by living parasites. Another murine monoclonal antibody (MAb-001), an IgG1, was produced against an outer surface 200 kDa membrane glycoprotein (Cs-gp200). This glycoprotein epitope is specific to C. salmositica , and attaches to the surface membrane by a phospholipid anchor. MAb-001 agglutinates the parasite but does not fix complement. The antibody inhibits the activities of both cysteine and metallo-proteases from the pathogen. Parasites coated with MAb-001 do not multiply in culture, and they die in about 3 weeks. The infectivity of antibody-coated parasite to fish was also significantly decreased. Also, MAb-001 injected intraperitoneally into fish with acute disease significantly lowered the parasitaemias (60–80%) within 48 h. This monoclonal antibody is species specific. The partial characterization of the glycoprotein epitope on Cs-gp200 is the first step towards the development of a subunit vaccine against the pathogen. Monoclonal antibody technology is useful, and it will likely be used more often in the future to study parasitic diseases of fish." @default.
• W2008396783 created "2016-06-24" @default.
• W2008396783 creator A5065485979 @default.
• W2008396783 date "1999-07-01" @default.
• W2008396783 modified "2023-09-27" @default.
• W2008396783 title "The use of monoclonal antibody probes in salmonid cryptobiosis" @default.
• W2008396783 cites W1594700207 @default.
• W2008396783 cites W1966781128 @default.
• W2008396783 cites W1966819069 @default.
• W2008396783 cites W1968748928 @default.
• W2008396783 cites W1973528554 @default.
• W2008396783 cites W1974594632 @default.
• W2008396783 cites W1979159863 @default.
• W2008396783 cites W1989481364 @default.
• W2008396783 cites W1994952636 @default.
• W2008396783 cites W1996996882 @default.
• W2008396783 cites W2005324031 @default.
• W2008396783 cites W2014790055 @default.
• W2008396783 cites W2020374274 @default.
• W2008396783 cites W2042201283 @default.
• W2008396783 cites W2046672966 @default.
• W2008396783 cites W2062581204 @default.
• W2008396783 cites W2069536777 @default.
• W2008396783 cites W2072931767 @default.
• W2008396783 cites W2079001244 @default.
• W2008396783 cites W2080117618 @default.
• W2008396783 cites W2089351814 @default.
• W2008396783 cites W2115025799 @default.
• W2008396783 cites W2115945549 @default.
• W2008396783 cites W2119187027 @default.
• W2008396783 cites W2120639491 @default.
• W2008396783 cites W2126305953 @default.
• W2008396783 cites W2138942321 @default.
• W2008396783 cites W2156916359 @default.
• W2008396783 cites W241648137 @default.
• W2008396783 cites W280875868 @default.
• W2008396783 cites W51219592 @default.
• W2008396783 cites W1966155834 @default.
• W2008396783 doi "https://doi.org/10.1016/s0044-8486(99)00094-0" @default.
• W2008396783 hasPublicationYear "1999" @default.
• W2008396783 type Work @default.
• W2008396783 sameAs 2008396783 @default.
• W2008396783 citedByCount "1" @default.
• W2008396783 crossrefType "journal-article" @default.
• W2008396783 hasAuthorship W2008396783A5065485979 @default.
• W2008396783 hasConcept C106358424 @default.
• W2008396783 hasConcept C108625454 @default.
• W2008396783 hasConcept C136764020 @default.
• W2008396783 hasConcept C147483822 @default.
• W2008396783 hasConcept C153911025 @default.
• W2008396783 hasConcept C159047783 @default.
• W2008396783 hasConcept C159654299 @default.
• W2008396783 hasConcept C195616568 @default.
• W2008396783 hasConcept C203014093 @default.
• W2008396783 hasConcept C22070199 @default.
• W2008396783 hasConcept C2522874641 @default.
• W2008396783 hasConcept C2776460866 @default.
• W2008396783 hasConcept C41008148 @default.
• W2008396783 hasConcept C542903549 @default.
• W2008396783 hasConcept C71928629 @default.
• W2008396783 hasConcept C86803240 @default.
• W2008396783 hasConcept C89423630 @default.
• W2008396783 hasConceptScore W2008396783C106358424 @default.
• W2008396783 hasConceptScore W2008396783C108625454 @default.
• W2008396783 hasConceptScore W2008396783C136764020 @default.
• W2008396783 hasConceptScore W2008396783C147483822 @default.
• W2008396783 hasConceptScore W2008396783C153911025 @default.
• W2008396783 hasConceptScore W2008396783C159047783 @default.
• W2008396783 hasConceptScore W2008396783C159654299 @default.
• W2008396783 hasConceptScore W2008396783C195616568 @default.
• W2008396783 hasConceptScore W2008396783C203014093 @default.
• W2008396783 hasConceptScore W2008396783C22070199 @default.
• W2008396783 hasConceptScore W2008396783C2522874641 @default.
• W2008396783 hasConceptScore W2008396783C2776460866 @default.
• W2008396783 hasConceptScore W2008396783C41008148 @default.
• W2008396783 hasConceptScore W2008396783C542903549 @default.
• W2008396783 hasConceptScore W2008396783C71928629 @default.
• W2008396783 hasConceptScore W2008396783C86803240 @default.
• W2008396783 hasConceptScore W2008396783C89423630 @default.
• W2008396783 hasLocation W20083967831 @default.
• W2008396783 hasOpenAccess W2008396783 @default.
• W2008396783 hasPrimaryLocation W20083967831 @default.
• W2008396783 hasRelatedWork W1917691850 @default.
• W2008396783 hasRelatedWork W1966185179 @default.
• W2008396783 hasRelatedWork W1979262146 @default.
• W2008396783 hasRelatedWork W1991729558 @default.
• W2008396783 hasRelatedWork W1994932839 @default.
• W2008396783 hasRelatedWork W2012622604 @default.
• W2008396783 hasRelatedWork W2022111735 @default.
• W2008396783 hasRelatedWork W2022815666 @default.
• W2008396783 hasRelatedWork W2032811226 @default.
• W2008396783 hasRelatedWork W2035388249 @default.
• W2008396783 hasRelatedWork W2039527543 @default.
• W2008396783 hasRelatedWork W2047889759 @default.
• W2008396783 hasRelatedWork W2067379048 @default.
• W2008396783 hasRelatedWork W2089351814 @default.
• W2008396783 hasRelatedWork W2092285206 @default.
• W2008396783 hasRelatedWork W2115020214 @default.
• W2008396783 hasRelatedWork W2150764812 @default.
• W2008396783 hasRelatedWork W2151806374 @default.

• next