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Matches in SemOpenAlex for { <https://semopenalex.org/work/W2008434921> ?p ?o ?g. }

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• W2008434921 abstract "We successfully cloned and expressed a single-chain antibody (425scFv), that is directed to human epidermal growth factor receptor HER1 (EGFR) in transgenic tobacco plants as a fusion with bacterial barstar gene (425scFv-barstar). Plant-produced recombinant 425scFv-barstar was recovered using barstar-barnase system. Based on barstar-barnase affinity, during purification of the plant-produced 425scFv-barstar, we generated bispecific scFv-antibody heterodimers from individual single-chain fragments initially produced in different host systems with binding activity to both HER1 and HER2/neu tumor antigens. We demonstrated by flow cytometry and indirect immunofluorescent microscopy that both the components of heterodimer retain its specific cell-binding activity." @default.
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• W2008434921 date "2007-01-01" @default.
• W2008434921 modified "2023-10-14" @default.
• W2008434921 title "Expression of single-chain antibody-barstar fusion in plants" @default.
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• W2008434921 doi "https://doi.org/10.1016/j.biochi.2006.07.012" @default.
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