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- W2008439689 abstract "Temperature-dependency of poly(U)-directed polyphenylalanine synthesis was examined with different cell-free systems of Vibrio sp. strain ABE-1, a psychrophile, and Pseudomonas aeruginosa, a mesophile. In S-30 fractions, the activity of V. ABE-1 was higher than that of P. aeruginosa at and below 25°, but the activity of the former decreased rapidly above 25° and was completely lost at 40°. In ribosome-supernatant systems, V. ABE-1 ribosomes, especially 50S subunits, served a cold-stabilizing function, but V. ABE-1 supernatant was remarkably thermolabile, which was probably attributable to thermo-inactivation of phenylalanyl-tRNA synthetase present in the supernatant. V. ABE-1 ribosomes had no activation factor able to be released by washing with 1M NH4Cl." @default.
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- W2008439689 date "1980-01-01" @default.
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- W2008439689 title "Effect of temperature on the cell-free protein-synthesizing system in psychrophilic and mesophilic bacteria." @default.
- W2008439689 doi "https://doi.org/10.2323/jgam.26.265" @default.
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