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- W2008440874 abstract "The induction of several biochemical indicators of larval tissue histolysis in the Medfly, Ceratitis capitata (Diptera: Tephritidae) (Wiedemann) was studied. Using synchronized third instar larvae, we have determined the time of occurence of gut evacuation (12 h before puparium formation, bpf), disappearance of digestive enzymes (10 h bpf), and jumping from the food (8 h bpf). We can also correlate these events temporally with other early landmarks of metamorphosis. The decrease in protein content between 0 hours and 144 hours after puparium formation (0–144 h apf) corresponds to a sharp increase of total acid proteolytic activity measured in vitro from 0 to 44 h apf. This activity appears to be lysosomal, judging by the activation of other lysosomal markers, such as acid phosphatase and β-glycosidases. The maximum proteolytic activity occurs during the pre-pupa to pupa transition, i.e. during morphogenesis from the cryptocephalic to the phanerocephalic pupa. The results of endopeptidase inhibitor assays indicate that in dipterans, members of the aspartic and cystein proteinase families are responsible for the degradation of larval tissues." @default.
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- W2008440874 date "2000-01-01" @default.
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- W2008440874 title "Metamorphosis-associated proteolysis in Ceratitis capitata" @default.
- W2008440874 doi "https://doi.org/10.1046/j.1570-7458.2000.00604.x" @default.
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