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- W2008451830 abstract "Gram-positive bacteria assemble pili through class C sortase enzymes specialized in polymerizing pilin subunits into covalently linked, high-molecular-weight, elongated structures. Here we report the crystal structures of two class C sortases (SrtC1 and SrtC2) from Group B Streptococcus (GBS) Pilus Island 1. The structures show that both sortases are comprised of two domains: an 8-stranded β-barrel catalytic core conserved among all sortase family members and a flexible N-terminal region made of two α-helices followed by a loop, known as the lid, which acts as a pseudo-substrate. In vitro experiments performed with recombinant SrtC enzymes lacking the N-terminal portion demonstrate that this region of the enzyme is dispensable for catalysis but may have key roles in substrate specificity and regulation. Moreover, in vitro FRET-based assays show that the LPXTG motif common to many sortase substrates is not the sole determinant of sortase C specificity during pilin protein recognition." @default.
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- W2008451830 date "2012-11-08" @default.
- W2008451830 modified "2023-09-27" @default.
- W2008451830 title "Structural Basis for Group B Streptococcus Pilus 1 Sortases C Regulation and Specificity" @default.
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- W2008451830 doi "https://doi.org/10.1371/journal.pone.0049048" @default.
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