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• W2008457375 abstract "The fractional volume occupied by extracellular space in tissues, termed α, is an important parameter of tissue architecture that affects cellular functions and drug delivery. We report a technically simple fluorescent dye partitioning method to measure α in tissue slices based on microfiberoptic detection of dye fluorescence in tissue versus overlying solution. Microfiberoptic tip geometry and dyes were selected for α determination from fluorescence intensity ratios, without the need to correct for illumination profile, light scattering/absorption, or dye binding. The method was validated experimentally using cell-embedded gels of specified α-values and optical properties. In mouse brain slices, α was strongly location-dependent, ranging from 0.16 in thalamus to 0.22 in brainstem, and was sensitive to cell volume changes. Aquaporin-4 water channel gene deletion caused significant extracellular space expansion, with α = 0.181 ± 0.002 in cortex in wild-type mice and 0.211 ± 0.003 in Aquaporin-4 knockout mice. In slices of LLC1 cell tumors grown in mice to ∼5 mm diameter, α decreased remarkably from ∼0.45 in superficial tumor to <0.25 in deeper (>100 μm) tumor. Fluorescent dye partitioning with microfiberoptic detection permits rapid, accurate, and anisotropy-insensitive determination of α-values in tissue slices." @default.
• W2008457375 created "2016-06-24" @default.
• W2008457375 creator A5042356327 @default.
• W2008457375 creator A5056450584 @default.
• W2008457375 date "2010-08-01" @default.
• W2008457375 modified "2023-09-27" @default.
• W2008457375 title "Microfiberoptic Measurement of Extracellular Space Volume in Brain and Tumor Slices Based on Fluorescent Dye Partitioning" @default.
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• W2008457375 doi "https://doi.org/10.1016/j.bpj.2010.06.023" @default.
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