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• W2008491189 abstract "A metallodendrimer-based electrochemical DNA biosensor was constructed by a layer-by-layer assembly of cobalt(II) salicylaldiimine metallodendrimer (SDD–Co(II)) and a 21 bases oligonucleotide NH2-5′-GAGGAGTTGGGGGAGCACATT-3′ (pDNA) on a gold electrode. The complementary oligonucleotide was 5′-AATGTGCTCCCCCAACTCCTC-3′ (tDNA). UV–visible spectra of SDD–Co(II) in 1:1 (v/v) acetone–ethanol solution showed absorption bands at 325 nm and 365–420 nm related to π–π* intra-dendrimer transitions and d–π* metal-dendrimer charge transfer transitions, respectively. Square-wave voltammetry (SWV) characterisation of the Au|SDD–Co(II)|pDNA biosensor system in phosphate buffer saline solution of pH 7.4, indicated a reversible one-electron electrochemical process with a formal potential, E°′, value of +210 mV. Electrochemical impedance spectroscopy (EIS) data confirmed that the hybridisation of the biosensor's pDNA with the tDNA to form double-stranded DNA (dsDNA) resulted in an increase of the impedimetric charge transfer resistance, Rct, value from 6.52 to 12.85 kΩ. The limit of detection (LOD), calculated as 3σ of the background noise, and sensitivity of the sensor were 1.29 kΩ/nmol L−1 and 0.34 pmol L−1, respectively." @default.
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• W2008491189 date "2010-05-01" @default.
• W2008491189 modified "2023-09-27" @default.
• W2008491189 title "Electrochemical and spectroscopic properties of dendritic cobalto-salicylaldiimine DNA biosensor" @default.
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• W2008491189 doi "https://doi.org/10.1016/j.electacta.2009.06.065" @default.
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