Matches in SemOpenAlex for { <https://semopenalex.org/work/W2008496590> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W2008496590 abstract "Abstract 1. 1. An acidic protease was purified to electrophoretic homogeneity (34-fold purification, 7% yield) from epimastigotes of Trypanosoma cruzi, Tulahuen strain, Tul 2 stock. The enzyme is monomeric, with a molecular weight of about 60,000. 2. 2. The purified enzyme was able to use as substrate azocasein, casein, bovine serum albumin and hemoglobin; the highest activity was found with bovine serum albumin at pH 4.0. Soluble T. cruzi proteins were also used as substrates, with an optimum pH of about 3.0. 3. 3. The purified enzyme was rather thermostable; 50% of the enzyme activity was lost upon preincubation at 62°C for 10 min at pH 5.0. Accordingly, the “optimal” temperature for the reaction with azocasein as substrate was 60°C. 4. 4. The enzyme was strongly inhibited by the thiol reagents p-chloromercuribenzoate, phenolphthalein mercuric acetate and p-chloromercuriphenylsulfonate (I50 values of 1.0, 1.2 and 3.0 × 10−6M, respectively); thiol-containing compounds, such as 2-mercaptoethanol and reduced glutathione, activated the enzyme; the former was also able to reactivate the enzyme inhibited by the mercurials. N-α-p- Tosyl- l -lysine chloromethyl ketone and N-α-p- tosyl- l -phenylalanine chloromethylketone were also strong inhibitors (I50 of 1.2 × 10−6 and 1.7 × 10−5 M, respectively); 2-mercaptoethanol did not revert these inhibitions. 5. 5. The properties of the purified protease suggest that it may be the main factor responsible for the proteolysis of endogenous substrates that we have described in cell-free extracts of T. cruzi (Cazzulo, J. J. and Franke de Cazzulo, B. M. (1982) Experientia (Basel) 38, 1135–1137)." @default.
- W2008496590 created "2016-06-24" @default.
- W2008496590 creator A5008975238 @default.
- W2008496590 creator A5049091535 @default.
- W2008496590 creator A5066444687 @default.
- W2008496590 creator A5091408096 @default.
- W2008496590 date "1984-01-01" @default.
- W2008496590 modified "2023-09-27" @default.
- W2008496590 title "Purification and some properties of an acidic protease from epimastigotes of Trypanosoma cruzi" @default.
- W2008496590 cites W1625660792 @default.
- W2008496590 cites W1775749144 @default.
- W2008496590 cites W1890321828 @default.
- W2008496590 cites W1966497279 @default.
- W2008496590 cites W2009480854 @default.
- W2008496590 cites W2032169756 @default.
- W2008496590 cites W2044809585 @default.
- W2008496590 cites W2070687039 @default.
- W2008496590 cites W2076153370 @default.
- W2008496590 cites W2086679893 @default.
- W2008496590 cites W2111301002 @default.
- W2008496590 cites W2137302959 @default.
- W2008496590 doi "https://doi.org/10.1016/0305-0491(84)90282-7" @default.
- W2008496590 hasPublicationYear "1984" @default.
- W2008496590 type Work @default.
- W2008496590 sameAs 2008496590 @default.
- W2008496590 citedByCount "15" @default.
- W2008496590 countsByYear W20084965902012 @default.
- W2008496590 countsByYear W20084965902013 @default.
- W2008496590 countsByYear W20084965902015 @default.
- W2008496590 crossrefType "journal-article" @default.
- W2008496590 hasAuthorship W2008496590A5008975238 @default.
- W2008496590 hasAuthorship W2008496590A5049091535 @default.
- W2008496590 hasAuthorship W2008496590A5066444687 @default.
- W2008496590 hasAuthorship W2008496590A5091408096 @default.
- W2008496590 hasConcept C136764020 @default.
- W2008496590 hasConcept C181199279 @default.
- W2008496590 hasConcept C185592680 @default.
- W2008496590 hasConcept C18903297 @default.
- W2008496590 hasConcept C2776142715 @default.
- W2008496590 hasConcept C2776714187 @default.
- W2008496590 hasConcept C2777289219 @default.
- W2008496590 hasConcept C2777625466 @default.
- W2008496590 hasConcept C2781307694 @default.
- W2008496590 hasConcept C2781324293 @default.
- W2008496590 hasConcept C28212737 @default.
- W2008496590 hasConcept C41008148 @default.
- W2008496590 hasConcept C43617362 @default.
- W2008496590 hasConcept C55493867 @default.
- W2008496590 hasConcept C71928629 @default.
- W2008496590 hasConcept C86803240 @default.
- W2008496590 hasConcept C86807702 @default.
- W2008496590 hasConceptScore W2008496590C136764020 @default.
- W2008496590 hasConceptScore W2008496590C181199279 @default.
- W2008496590 hasConceptScore W2008496590C185592680 @default.
- W2008496590 hasConceptScore W2008496590C18903297 @default.
- W2008496590 hasConceptScore W2008496590C2776142715 @default.
- W2008496590 hasConceptScore W2008496590C2776714187 @default.
- W2008496590 hasConceptScore W2008496590C2777289219 @default.
- W2008496590 hasConceptScore W2008496590C2777625466 @default.
- W2008496590 hasConceptScore W2008496590C2781307694 @default.
- W2008496590 hasConceptScore W2008496590C2781324293 @default.
- W2008496590 hasConceptScore W2008496590C28212737 @default.
- W2008496590 hasConceptScore W2008496590C41008148 @default.
- W2008496590 hasConceptScore W2008496590C43617362 @default.
- W2008496590 hasConceptScore W2008496590C55493867 @default.
- W2008496590 hasConceptScore W2008496590C71928629 @default.
- W2008496590 hasConceptScore W2008496590C86803240 @default.
- W2008496590 hasConceptScore W2008496590C86807702 @default.
- W2008496590 hasLocation W20084965901 @default.
- W2008496590 hasOpenAccess W2008496590 @default.
- W2008496590 hasPrimaryLocation W20084965901 @default.
- W2008496590 hasRelatedWork W1549352256 @default.
- W2008496590 hasRelatedWork W158525432 @default.
- W2008496590 hasRelatedWork W193277563 @default.
- W2008496590 hasRelatedWork W1963773112 @default.
- W2008496590 hasRelatedWork W1972000677 @default.
- W2008496590 hasRelatedWork W1974355562 @default.
- W2008496590 hasRelatedWork W1991957381 @default.
- W2008496590 hasRelatedWork W1995639681 @default.
- W2008496590 hasRelatedWork W2009480854 @default.
- W2008496590 hasRelatedWork W2014174038 @default.
- W2008496590 hasRelatedWork W2045572833 @default.
- W2008496590 hasRelatedWork W2053277946 @default.
- W2008496590 hasRelatedWork W2055329059 @default.
- W2008496590 hasRelatedWork W2070687039 @default.
- W2008496590 hasRelatedWork W2086679893 @default.
- W2008496590 hasRelatedWork W2100837269 @default.
- W2008496590 hasRelatedWork W2113510735 @default.
- W2008496590 hasRelatedWork W2128635872 @default.
- W2008496590 hasRelatedWork W2164295778 @default.
- W2008496590 hasRelatedWork W2419185330 @default.
- W2008496590 isParatext "false" @default.
- W2008496590 isRetracted "false" @default.
- W2008496590 magId "2008496590" @default.
- W2008496590 workType "article" @default.