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- W2008507002 abstract "Abstract A developed method was used for the enhancement of arachidonic acid production by M. isabellina . An orthogonal, rotatable and central composite design was applied to determine the optimum conditions for protoplast regeneration mutagenesis. The results showed that a commixture enzyme (cellulase and glusulase) at the concentration of 4%, enzymolysis temperature at 30°C and enzymolysis time on 7.5 h were the optimal conditions, in which the lethality of M. isabellina spores was 78.4%. After mutagenesis and re-screenings, M. isabellina mutant Y-69 was obtained. GC analysis showed that the yield of arachidonic acid by Y-69 (2.92 g·L −1 ) was 3.56 times higher than that of the wild-type strain (0.82 g · L −1 ). Pass generation tests showed that the properties of Y-69 by mutation were readily inherited." @default.
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- W2008507002 date "2011-06-01" @default.
- W2008507002 modified "2023-09-25" @default.
- W2008507002 title "Enhancement of Arachidonic Acid Production by Mortierella isabellina Through Protoplast Regeneration Mutagenesis" @default.
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- W2008507002 doi "https://doi.org/10.1016/s1006-8104(12)60012-9" @default.
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