FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2008511275> ?p ?o ?g. }

• W2008511275 abstract "hnRNP K, a member of the family of heterogeneous ribonucleoproteins, is known to exert various functional roles in the nucleus, cytoplasm, and mitochondria to affect different cellular processes including chromatin remodeling, transcription, splicing, and translation. Here we report, for the first time, that hnRNP K is specifically involved in human LDL receptor (LDLR) gene transcription in HepG2 cells. We show that depletion of hnRNP K by siRNA transfection reduces the expression of LDLR mRNA and protein by more than 50% as measured by quantitative real-time PCR and Western blot analysis. Importantly, we show that the decay rate of LDLR mRNA is not affected by hnRNP K siRNA transfection, whereas the LDLR promoter activity is significantly decreased. Furthermore, overexpression of hnRNP K increased the LDLR promoter activity by the luciferase reporter assay. By utilizing a series of mutational and deletional constructs of LDLR promoter luciferase reporters, we mapped the K-responsive element to the repeat 3 (R3) sequence of the LDLR promoter. Electrophoretic mobility shift assays show that the K protein binds to a single-stranded DNA probe containing the CT-rich element of R3, which is in contrast to the requirement of double-stranded DNA for Sp1 to bind to R3. Finally, chromatin immunoprecipitation assays reveal a direct interaction of hnRNP K with the LDLR promoter in intact HepG2 cells. These new findings provide strong evidence demonstrating that hnRNP K is an important transactivator for human LDLR gene transcription. This work sheds new light on our current understanding of how LDLR gene expression is controlled at the transcriptional level." @default.
• W2008511275 created "2016-06-24" @default.
• W2008511275 creator A5067619955 @default.
• W2008511275 creator A5070924852 @default.
• W2008511275 date "2010-06-01" @default.
• W2008511275 modified "2023-10-03" @default.
• W2008511275 title "Identification of Heterogeneous Nuclear Ribonucleoprotein K as a Transactivator for Human Low Density Lipoprotein Receptor Gene Transcription" @default.
• W2008511275 cites W1522779995 @default.
• W2008511275 cites W1589947860 @default.
• W2008511275 cites W1658669968 @default.
• W2008511275 cites W1965676778 @default.
• W2008511275 cites W1970346631 @default.
• W2008511275 cites W1976865161 @default.
• W2008511275 cites W1979607371 @default.
• W2008511275 cites W1980449471 @default.
• W2008511275 cites W1994701090 @default.
• W2008511275 cites W1997266292 @default.
• W2008511275 cites W2005893109 @default.
• W2008511275 cites W2013128876 @default.
• W2008511275 cites W2013741637 @default.
• W2008511275 cites W2025817543 @default.
• W2008511275 cites W2026119296 @default.
• W2008511275 cites W2026937822 @default.
• W2008511275 cites W2041189065 @default.
• W2008511275 cites W2045319408 @default.
• W2008511275 cites W2056411372 @default.
• W2008511275 cites W2064877960 @default.
• W2008511275 cites W2069136255 @default.
• W2008511275 cites W2073229191 @default.
• W2008511275 cites W2078300866 @default.
• W2008511275 cites W2089425542 @default.
• W2008511275 cites W2092265778 @default.
• W2008511275 cites W2093201670 @default.
• W2008511275 cites W2095264993 @default.
• W2008511275 cites W2104902761 @default.
• W2008511275 cites W2112115500 @default.
• W2008511275 cites W2113031608 @default.
• W2008511275 cites W2113442590 @default.
• W2008511275 cites W2127791131 @default.
• W2008511275 cites W2129582474 @default.
• W2008511275 cites W2137319242 @default.
• W2008511275 cites W2137625554 @default.
• W2008511275 cites W2141238215 @default.
• W2008511275 cites W2148015985 @default.
• W2008511275 cites W2152804961 @default.
• W2008511275 cites W2152957900 @default.
• W2008511275 cites W2163930739 @default.
• W2008511275 cites W2170397176 @default.
• W2008511275 cites W4243500197 @default.
• W2008511275 doi "https://doi.org/10.1074/jbc.m109.082057" @default.
• W2008511275 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2878543" @default.
• W2008511275 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20371611" @default.
• W2008511275 hasPublicationYear "2010" @default.
• W2008511275 type Work @default.
• W2008511275 sameAs 2008511275 @default.
• W2008511275 citedByCount "20" @default.
• W2008511275 countsByYear W20085112752012 @default.
• W2008511275 countsByYear W20085112752013 @default.
• W2008511275 countsByYear W20085112752014 @default.
• W2008511275 countsByYear W20085112752015 @default.
• W2008511275 countsByYear W20085112752017 @default.
• W2008511275 countsByYear W20085112752019 @default.
• W2008511275 countsByYear W20085112752021 @default.
• W2008511275 countsByYear W20085112752023 @default.
• W2008511275 crossrefType "journal-article" @default.
• W2008511275 hasAuthorship W2008511275A5067619955 @default.
• W2008511275 hasAuthorship W2008511275A5070924852 @default.
• W2008511275 hasBestOaLocation W20085112751 @default.
• W2008511275 hasConcept C101762097 @default.
• W2008511275 hasConcept C104317684 @default.
• W2008511275 hasConcept C1292079 @default.
• W2008511275 hasConcept C134320426 @default.
• W2008511275 hasConcept C138885662 @default.
• W2008511275 hasConcept C150194340 @default.
• W2008511275 hasConcept C153911025 @default.
• W2008511275 hasConcept C159194896 @default.
• W2008511275 hasConcept C179926584 @default.
• W2008511275 hasConcept C183978625 @default.
• W2008511275 hasConcept C2778163477 @default.
• W2008511275 hasConcept C2780072125 @default.
• W2008511275 hasConcept C2909472194 @default.
• W2008511275 hasConcept C41895202 @default.
• W2008511275 hasConcept C43554185 @default.
• W2008511275 hasConcept C54355233 @default.
• W2008511275 hasConcept C55493867 @default.
• W2008511275 hasConcept C67705224 @default.
• W2008511275 hasConcept C75934600 @default.
• W2008511275 hasConcept C83640560 @default.
• W2008511275 hasConcept C86339819 @default.
• W2008511275 hasConcept C86803240 @default.
• W2008511275 hasConceptScore W2008511275C101762097 @default.
• W2008511275 hasConceptScore W2008511275C104317684 @default.
• W2008511275 hasConceptScore W2008511275C1292079 @default.
• W2008511275 hasConceptScore W2008511275C134320426 @default.
• W2008511275 hasConceptScore W2008511275C138885662 @default.
• W2008511275 hasConceptScore W2008511275C150194340 @default.
• W2008511275 hasConceptScore W2008511275C153911025 @default.
• W2008511275 hasConceptScore W2008511275C159194896 @default.
• W2008511275 hasConceptScore W2008511275C179926584 @default.
• W2008511275 hasConceptScore W2008511275C183978625 @default.

• next