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• W2008528050 abstract "Schizochytrium limacinum SR21 was used to remove waste nutrients from a marine recirculating aquaculture wastewater while simultaneously producing a valuable biomass containing PUFA enriched with long chain fatty acids which may have potential as an aquafeed additive. Clean seawater and marine aquaculture wastewater supplemented with yeast extract (1.0–10 g L−1) and glycerol (10–90 g L−1) were used for a comparative study of growth and PUFA production using S. limacinum in batch, continuous and in a packed bed reactor (pall rings, surface area 340 m2/m3) that allow surface growth and cell recycling for a high cell density culture. Good growth rates (μ = 0.013 h−1) were obtained in batch and continuous culture modes. Biomass of S. limacinum was increased from 4.0 g L−1 as DCW in batch culture to 57.9 g L−1 in retention culture (D = 0.062 h−1). Retention culture of S. limacinum in a packed bed reactor also produced total lipids of 36.5 g L−1 (53–65%, w/w as DCW) and long chain fatty acids (>C22) of 12.6 g L−1 (21.8%, w/w as DCW) in 350 h. Long chain fatty acids of C22, C18 and C16, as the main fatty acids, were produced and production was enhanced when supplemented wastewater was used in growth medium. Changes to the relative concentrations of glycerol and yeast extract (C:N ratio) alter the amounts of oil produced and types of fatty acid produced. S. limacinum in continuous culture mode (D = 0.013 h−1) enabled significant reductions (<90%) in phosphorous, nitrogen and ammonia concentrations of marine aquaculture wastewater and efficiency of wastewater treatment was further improved in retention culture operating at D = 0.062 h−1. This system would be well suited to treating nutrient concentrated effluent from sludge sedimentation/dewatering tanks in recirculating aquaculture systems (RAS). The treatment capacity of the reactor was considerable and in this current study, 420 g N, 190 g P and 10 g total NH3 day−1 m−3 reactor could be removed using the retention culture system." @default.
• W2008528050 created "2016-06-24" @default.
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• W2008528050 date "2010-09-01" @default.
• W2008528050 modified "2023-09-26" @default.
• W2008528050 title "Integrated production of long chain polyunsaturated fatty acids (PUFA)-rich Schizochytrium biomass using a nutrient supplemented marine aquaculture wastewater" @default.
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• W2008528050 doi "https://doi.org/10.1016/j.aquaeng.2010.05.004" @default.
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