Matches in SemOpenAlex for { <https://semopenalex.org/work/W2008558883> ?p ?o ?g. }
Showing items 1 to 74 of
74
with 100 items per page.
- W2008558883 endingPage "316a" @default.
- W2008558883 startingPage "316a" @default.
- W2008558883 abstract "Deletion or mutation of a variety of proteins localized at cell-matrix and cell-cell junctions, such as vinculin and its splice-variant metavinculin, can lead to dilated cardiomyopathy in mice and humans, leading some to hypothesize that these molecules are involved in mechanotransmission or mechanotransduction in the heart.To investigate cardiac mechanotransduction mechanisms in single cells, we have combined laser tweezers with a fluorescence resonance energy transfer (FRET) biosensor to apply localized forces and probe localized signaling events in isolated mouse ventricular myocytes.Isolated murine ventricular cardiac myocytes were transfected with a focal adhesion kinase (FAK) FRET reporter to monitor integrin-mediated activation events. An integrin ligand-coated microsphere was adhered to the cell surface. Then laser tweezers were used to apply localized piconewton forces parallel or normal to the image plane either cyclically or statically. To ensure a constant force application on the microsphere, 10υM of blebbistatin was added to the imaging media. In conjunction with force application, a pulsed Ti:sapphire infrared laser was used for two-photon excitation of the FRET reporter.Phase contrast and fluorescent images were captured simultaneously, allowing quantification of applied forces and FRET ratio changes. Results indicate that piconewton level forces can be applied to the microsphere and FRET ratio changes validate that integrin-mediated events are being activated by the locally applied forces. This data suggests that the use of laser tweezers combined with FRET provides a means to study integrin-mediated events in cardiac mechanotransduction. Future studies include varying the type of integrin ligand-coated microsphere and FRET biosensors, as well as studying genetically manipulated murine lines in isolated adult cardiac myocytes." @default.
- W2008558883 created "2016-06-24" @default.
- W2008558883 creator A5033811596 @default.
- W2008558883 creator A5049101165 @default.
- W2008558883 creator A5066337260 @default.
- W2008558883 creator A5075423154 @default.
- W2008558883 creator A5084427519 @default.
- W2008558883 date "2009-02-01" @default.
- W2008558883 modified "2023-09-27" @default.
- W2008558883 title "Mechanotransduction in Single Cardiac Myocyte Studied Using Laser Tweezers and FRET" @default.
- W2008558883 doi "https://doi.org/10.1016/j.bpj.2008.12.1582" @default.
- W2008558883 hasPublicationYear "2009" @default.
- W2008558883 type Work @default.
- W2008558883 sameAs 2008558883 @default.
- W2008558883 citedByCount "1" @default.
- W2008558883 crossrefType "journal-article" @default.
- W2008558883 hasAuthorship W2008558883A5033811596 @default.
- W2008558883 hasAuthorship W2008558883A5049101165 @default.
- W2008558883 hasAuthorship W2008558883A5066337260 @default.
- W2008558883 hasAuthorship W2008558883A5075423154 @default.
- W2008558883 hasAuthorship W2008558883A5084427519 @default.
- W2008558883 hasBestOaLocation W20085588831 @default.
- W2008558883 hasConcept C120665830 @default.
- W2008558883 hasConcept C121332964 @default.
- W2008558883 hasConcept C12554922 @default.
- W2008558883 hasConcept C1491633281 @default.
- W2008558883 hasConcept C181104049 @default.
- W2008558883 hasConcept C185592680 @default.
- W2008558883 hasConcept C195687474 @default.
- W2008558883 hasConcept C20198109 @default.
- W2008558883 hasConcept C207200792 @default.
- W2008558883 hasConcept C55493867 @default.
- W2008558883 hasConcept C86803240 @default.
- W2008558883 hasConcept C91881484 @default.
- W2008558883 hasConcept C95444343 @default.
- W2008558883 hasConcept C96305047 @default.
- W2008558883 hasConceptScore W2008558883C120665830 @default.
- W2008558883 hasConceptScore W2008558883C121332964 @default.
- W2008558883 hasConceptScore W2008558883C12554922 @default.
- W2008558883 hasConceptScore W2008558883C1491633281 @default.
- W2008558883 hasConceptScore W2008558883C181104049 @default.
- W2008558883 hasConceptScore W2008558883C185592680 @default.
- W2008558883 hasConceptScore W2008558883C195687474 @default.
- W2008558883 hasConceptScore W2008558883C20198109 @default.
- W2008558883 hasConceptScore W2008558883C207200792 @default.
- W2008558883 hasConceptScore W2008558883C55493867 @default.
- W2008558883 hasConceptScore W2008558883C86803240 @default.
- W2008558883 hasConceptScore W2008558883C91881484 @default.
- W2008558883 hasConceptScore W2008558883C95444343 @default.
- W2008558883 hasConceptScore W2008558883C96305047 @default.
- W2008558883 hasIssue "3" @default.
- W2008558883 hasLocation W20085588831 @default.
- W2008558883 hasOpenAccess W2008558883 @default.
- W2008558883 hasPrimaryLocation W20085588831 @default.
- W2008558883 hasRelatedWork W1545635052 @default.
- W2008558883 hasRelatedWork W1998517356 @default.
- W2008558883 hasRelatedWork W2004508563 @default.
- W2008558883 hasRelatedWork W2014249634 @default.
- W2008558883 hasRelatedWork W2090409579 @default.
- W2008558883 hasRelatedWork W2094935699 @default.
- W2008558883 hasRelatedWork W2115150619 @default.
- W2008558883 hasRelatedWork W2229962237 @default.
- W2008558883 hasRelatedWork W2559863449 @default.
- W2008558883 hasRelatedWork W2914457341 @default.
- W2008558883 hasRelatedWork W2942736936 @default.
- W2008558883 hasRelatedWork W3082947114 @default.
- W2008558883 hasRelatedWork W3099975265 @default.
- W2008558883 hasVolume "96" @default.
- W2008558883 isParatext "false" @default.
- W2008558883 isRetracted "false" @default.
- W2008558883 magId "2008558883" @default.
- W2008558883 workType "article" @default.