Matches in SemOpenAlex for { <https://semopenalex.org/work/W2008589078> ?p ?o ?g. }
Showing items 1 to 80 of
80
with 100 items per page.
- W2008589078 endingPage "81" @default.
- W2008589078 startingPage "75" @default.
- W2008589078 abstract "One of the methods for calibration of antigen specific antibodies in a serum involves parallel titration of known concentrations of purified normal immunoglobulins (Igs) and a specific antiserum. We evaluated the effect of three methods for capturing known concentrations of purified normal mouse Igs (IgG, IgG subclasses and IgM) on the anti-tetanus toxoid antibody concentrations assigned to a reference mouse serum: (1) direct coating, (2) capture by pre-coated anti-mouse IgG (Fc) specific antibodies, and (3) capture by pre-coated anti-mouse IgG (Fab) specific antibodies. Direct coating of purified igs onto plastic was the least efficient and would greatly overestimate antigen specific antibody concentrations. Equivalent concentrations of purified IgG subclasses and IgM produced higher absorbances when captured by anti-Fab antibodies pre-coated on plates than by anti-Fc antibodies. As a consequence, the anti-Fc capture method resulted in the assignment of IgG1, IgG2a and IgG2b tetanus specific antibody concentrations which exceeded the total IgG1, IgG2a and IgG2b concentrations. Additionally, the sum of tetanus specific IgG subclass antibodies exceeded the tetanus specific IgG antibody concentration by >2-fold. In contrast, the anti-Fab capture method resulted in 2–9-fold lower assigned tetanus antibody concentrations which did not exceed the total Ig concentrations. We conclude that when using enzyme-linked immunosorbent assays, parallel titration of purified normal Ig standards captured by anti-Fab antibodies is superior to anti-Fc capture or direct coating for calibrating antigen specific antibodies in a reference serum." @default.
- W2008589078 created "2016-06-24" @default.
- W2008589078 creator A5013602212 @default.
- W2008589078 creator A5090330371 @default.
- W2008589078 date "1995-04-01" @default.
- W2008589078 modified "2023-09-28" @default.
- W2008589078 title "Method for quantitation of IgG subclass antibodies in mouse serum by enzyme-linked immunosorbent assay" @default.
- W2008589078 cites W1967200361 @default.
- W2008589078 cites W1984964459 @default.
- W2008589078 cites W2018082716 @default.
- W2008589078 cites W2032127941 @default.
- W2008589078 cites W2044123277 @default.
- W2008589078 cites W2120286442 @default.
- W2008589078 doi "https://doi.org/10.1016/0022-1759(94)00331-p" @default.
- W2008589078 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7730666" @default.
- W2008589078 hasPublicationYear "1995" @default.
- W2008589078 type Work @default.
- W2008589078 sameAs 2008589078 @default.
- W2008589078 citedByCount "36" @default.
- W2008589078 countsByYear W20085890782012 @default.
- W2008589078 countsByYear W20085890782013 @default.
- W2008589078 countsByYear W20085890782014 @default.
- W2008589078 countsByYear W20085890782015 @default.
- W2008589078 countsByYear W20085890782018 @default.
- W2008589078 countsByYear W20085890782019 @default.
- W2008589078 countsByYear W20085890782020 @default.
- W2008589078 countsByYear W20085890782023 @default.
- W2008589078 crossrefType "journal-article" @default.
- W2008589078 hasAuthorship W2008589078A5013602212 @default.
- W2008589078 hasAuthorship W2008589078A5090330371 @default.
- W2008589078 hasConcept C147483822 @default.
- W2008589078 hasConcept C153911025 @default.
- W2008589078 hasConcept C159654299 @default.
- W2008589078 hasConcept C185592680 @default.
- W2008589078 hasConcept C203014093 @default.
- W2008589078 hasConcept C22070199 @default.
- W2008589078 hasConcept C2776194381 @default.
- W2008589078 hasConcept C2776612079 @default.
- W2008589078 hasConcept C2777351567 @default.
- W2008589078 hasConcept C2779841045 @default.
- W2008589078 hasConcept C2780801004 @default.
- W2008589078 hasConcept C2780898057 @default.
- W2008589078 hasConcept C86803240 @default.
- W2008589078 hasConcept C91523082 @default.
- W2008589078 hasConceptScore W2008589078C147483822 @default.
- W2008589078 hasConceptScore W2008589078C153911025 @default.
- W2008589078 hasConceptScore W2008589078C159654299 @default.
- W2008589078 hasConceptScore W2008589078C185592680 @default.
- W2008589078 hasConceptScore W2008589078C203014093 @default.
- W2008589078 hasConceptScore W2008589078C22070199 @default.
- W2008589078 hasConceptScore W2008589078C2776194381 @default.
- W2008589078 hasConceptScore W2008589078C2776612079 @default.
- W2008589078 hasConceptScore W2008589078C2777351567 @default.
- W2008589078 hasConceptScore W2008589078C2779841045 @default.
- W2008589078 hasConceptScore W2008589078C2780801004 @default.
- W2008589078 hasConceptScore W2008589078C2780898057 @default.
- W2008589078 hasConceptScore W2008589078C86803240 @default.
- W2008589078 hasConceptScore W2008589078C91523082 @default.
- W2008589078 hasIssue "1" @default.
- W2008589078 hasLocation W20085890781 @default.
- W2008589078 hasLocation W20085890782 @default.
- W2008589078 hasOpenAccess W2008589078 @default.
- W2008589078 hasPrimaryLocation W20085890781 @default.
- W2008589078 hasRelatedWork W1522397301 @default.
- W2008589078 hasRelatedWork W1545596062 @default.
- W2008589078 hasRelatedWork W168309117 @default.
- W2008589078 hasRelatedWork W1834733894 @default.
- W2008589078 hasRelatedWork W1973692620 @default.
- W2008589078 hasRelatedWork W2008589078 @default.
- W2008589078 hasRelatedWork W2053168101 @default.
- W2008589078 hasRelatedWork W2315555091 @default.
- W2008589078 hasRelatedWork W4313309106 @default.
- W2008589078 hasRelatedWork W62158587 @default.
- W2008589078 hasVolume "181" @default.
- W2008589078 isParatext "false" @default.
- W2008589078 isRetracted "false" @default.
- W2008589078 magId "2008589078" @default.
- W2008589078 workType "article" @default.