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- W2008599030 abstract "The aim of this study was to investigate the effects of alginate and agarose on the response of bone marrow stromal cells (BMSCs) to chondrogenic stimuli. Rat BMSCs were expanded in monolayer culture with or without FGF-2 supplementation. Cells were then seeded in 2% alginate and agarose gels and cultured in media with or without TGF-β1 or dexamethasone (Dex). Sulfated glycosaminoglycans (sGAGs), collagen type II, and aggrecan were expressed in all groups that received TGF-β1 treatment during hydrogel culture. Expansion of rat BMSCs in the presence of FGF-2 increased production of sGAG in TGF-β1-treated groups over those cultures that were treated with TGF-β1 alone in alginate cultures. However, in agarose, cells exposed to FGF-2 during expansion produced less sGAG within TGF-β1-supplemented groups over those cultures treated with TGF-β1 alone. Dex was required for optimal matrix synthesis in both hydrogels, but was found to decrease cell viability in agarose constructs. These results indicate that the response of BMSCs to a chondrogenic growth factor regimen is scaffold dependent." @default.
- W2008599030 created "2016-06-24" @default.
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- W2008599030 date "2007-04-01" @default.
- W2008599030 modified "2023-10-17" @default.
- W2008599030 title "Hydrogel effects on bone marrow stromal cell response to chondrogenic growth factors" @default.
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- W2008599030 doi "https://doi.org/10.1016/j.biomaterials.2007.01.010" @default.
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