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• W2008601918 abstract "<i>Triportheus</i> fish species present 2n = 52 chromosomes. The karyotypes show similar macrostructure and a ZZ/ZW sex chromosome system, which probably represents synapomorphy for the genus. A probe of the Z chromosome was obtained from <i>T. nematurus</i> through microdissection, followed by unspecific amplification via DOP-PCR. This probe was used for WCP (whole chromosome painting) through fluorescent in situ hybridization (FISH) in several other <i>Triportheus </i>species, to analyze the differentiation of the ZZ/ZW system. The homology between this probe and chromosomes of species from other genera, putatively related to <i>Triportheus</i>, was also examined to search for evidence about evolution of their sex chromosomes. Complete homology was found among the Z chromosomes of all <i>Triportheus</i> species, while only small positive signals were found on the W chromosomes. Hybridization signals were absent in species from other genera. The present results reinforce both the conservative nature of Z chromosomes and the hypothesis that the ZZ/ZW sex chromosome system is a synapomorphic feature of <i>Triportheus</i>. On the other hand, besides reduction of size, W chromosomes have undergone accentuated composition changes in relation to Z chromosomes, since only a small region, usually located in the short arm, kept homology with the Z chromosomes." @default.
• W2008601918 created "2016-06-24" @default.
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• W2008601918 date "2008-01-01" @default.
• W2008601918 modified "2023-09-23" @default.
• W2008601918 title "Microdissection and whole chromosome painting. Improving sex chromosome analysis in <i>Triportheus</i> (Teleostei, Characiformes)" @default.
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• W2008601918 doi "https://doi.org/10.1159/000163094" @default.
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