Matches in SemOpenAlex for { <https://semopenalex.org/work/W2008643749> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W2008643749 endingPage "250" @default.
- W2008643749 startingPage "244" @default.
- W2008643749 abstract "The EE subunit of horse liver alcohol dehydrogenase (HLADH-EE) has been subcloned in pRSETb vector to generate a fusion His-tag protein. The migration from a multistep purification protocol for this well-known enzyme to a single-step has been successfully achieved. Several adjustments to the traditional purification procedure for His-tag proteins have been made to retain protein activity. A full characterization of the fusion enzyme has been carried out and compared with the native one. The K m for EtOH, NAD and NADH in the His-tag version of HLADH are in line with the ones reported in literature for the native enzyme. A shift in optimal pH activity is also observed. The enzyme retains the same stability and quaternary structure as the wild type and can therefore be easily used instead of the native HLADH for biotechnological applications." @default.
- W2008643749 created "2016-06-24" @default.
- W2008643749 creator A5025548733 @default.
- W2008643749 creator A5062263560 @default.
- W2008643749 creator A5071782166 @default.
- W2008643749 date "2012-04-28" @default.
- W2008643749 modified "2023-09-25" @default.
- W2008643749 title "Horse Liver Alcohol Dehydrogenase: New Perspectives for an Old Enzyme" @default.
- W2008643749 cites W1525724539 @default.
- W2008643749 cites W1533946522 @default.
- W2008643749 cites W1569170977 @default.
- W2008643749 cites W187777409 @default.
- W2008643749 cites W1971176983 @default.
- W2008643749 cites W1985506596 @default.
- W2008643749 cites W2013621384 @default.
- W2008643749 cites W2017816319 @default.
- W2008643749 cites W2036325431 @default.
- W2008643749 cites W2067230356 @default.
- W2008643749 cites W2082638707 @default.
- W2008643749 cites W2100837269 @default.
- W2008643749 cites W2106246537 @default.
- W2008643749 cites W2124404121 @default.
- W2008643749 cites W2128635872 @default.
- W2008643749 cites W2137450588 @default.
- W2008643749 cites W2138904529 @default.
- W2008643749 cites W243303449 @default.
- W2008643749 doi "https://doi.org/10.1007/s12033-012-9542-7" @default.
- W2008643749 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22544564" @default.
- W2008643749 hasPublicationYear "2012" @default.
- W2008643749 type Work @default.
- W2008643749 sameAs 2008643749 @default.
- W2008643749 citedByCount "24" @default.
- W2008643749 countsByYear W20086437492013 @default.
- W2008643749 countsByYear W20086437492014 @default.
- W2008643749 countsByYear W20086437492015 @default.
- W2008643749 countsByYear W20086437492016 @default.
- W2008643749 countsByYear W20086437492017 @default.
- W2008643749 countsByYear W20086437492018 @default.
- W2008643749 countsByYear W20086437492019 @default.
- W2008643749 countsByYear W20086437492020 @default.
- W2008643749 countsByYear W20086437492021 @default.
- W2008643749 countsByYear W20086437492022 @default.
- W2008643749 crossrefType "journal-article" @default.
- W2008643749 hasAuthorship W2008643749A5025548733 @default.
- W2008643749 hasAuthorship W2008643749A5062263560 @default.
- W2008643749 hasAuthorship W2008643749A5071782166 @default.
- W2008643749 hasBestOaLocation W20086437492 @default.
- W2008643749 hasConcept C104292427 @default.
- W2008643749 hasConcept C104317684 @default.
- W2008643749 hasConcept C123894998 @default.
- W2008643749 hasConcept C147816474 @default.
- W2008643749 hasConcept C151730666 @default.
- W2008643749 hasConcept C181199279 @default.
- W2008643749 hasConcept C185592680 @default.
- W2008643749 hasConcept C2777344606 @default.
- W2008643749 hasConcept C2780762169 @default.
- W2008643749 hasConcept C40767141 @default.
- W2008643749 hasConcept C43617362 @default.
- W2008643749 hasConcept C55493867 @default.
- W2008643749 hasConcept C75520062 @default.
- W2008643749 hasConcept C7927669 @default.
- W2008643749 hasConcept C86803240 @default.
- W2008643749 hasConceptScore W2008643749C104292427 @default.
- W2008643749 hasConceptScore W2008643749C104317684 @default.
- W2008643749 hasConceptScore W2008643749C123894998 @default.
- W2008643749 hasConceptScore W2008643749C147816474 @default.
- W2008643749 hasConceptScore W2008643749C151730666 @default.
- W2008643749 hasConceptScore W2008643749C181199279 @default.
- W2008643749 hasConceptScore W2008643749C185592680 @default.
- W2008643749 hasConceptScore W2008643749C2777344606 @default.
- W2008643749 hasConceptScore W2008643749C2780762169 @default.
- W2008643749 hasConceptScore W2008643749C40767141 @default.
- W2008643749 hasConceptScore W2008643749C43617362 @default.
- W2008643749 hasConceptScore W2008643749C55493867 @default.
- W2008643749 hasConceptScore W2008643749C75520062 @default.
- W2008643749 hasConceptScore W2008643749C7927669 @default.
- W2008643749 hasConceptScore W2008643749C86803240 @default.
- W2008643749 hasIssue "3" @default.
- W2008643749 hasLocation W20086437491 @default.
- W2008643749 hasLocation W20086437492 @default.
- W2008643749 hasLocation W20086437493 @default.
- W2008643749 hasOpenAccess W2008643749 @default.
- W2008643749 hasPrimaryLocation W20086437491 @default.
- W2008643749 hasRelatedWork W103497594 @default.
- W2008643749 hasRelatedWork W1510038873 @default.
- W2008643749 hasRelatedWork W2005799185 @default.
- W2008643749 hasRelatedWork W2008643749 @default.
- W2008643749 hasRelatedWork W2035523887 @default.
- W2008643749 hasRelatedWork W2055987780 @default.
- W2008643749 hasRelatedWork W2077691957 @default.
- W2008643749 hasRelatedWork W2145744708 @default.
- W2008643749 hasRelatedWork W2168275526 @default.
- W2008643749 hasRelatedWork W2395807491 @default.
- W2008643749 hasVolume "52" @default.
- W2008643749 isParatext "false" @default.
- W2008643749 isRetracted "false" @default.
- W2008643749 magId "2008643749" @default.
- W2008643749 workType "article" @default.