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- W2008648897 abstract "We describe a method to facilitate electrophoretic separation of high-molecular-weight RNA species, such as ribosomal RNAs and their precursors, on agarose–formaldehyde gels. Two alternative “pK-matched” buffer systems were substituted for the traditionally used Mops-based conductive medium. The key advantages include shortened run times, a 5-fold reduction in formaldehyde concentration, a significantly improved resolution of long RNAs, and consistency in separation. The new procedure has a streamlined work flow that helps to minimize errors and is broadly applicable to agarose gel electrophoresis of RNA samples and their subsequent analysis by Northern blotting." @default.
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- W2008648897 date "2013-10-01" @default.
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- W2008648897 title "Separation of long RNA by agarose–formaldehyde gel electrophoresis" @default.
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- W2008648897 doi "https://doi.org/10.1016/j.ab.2013.06.008" @default.
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