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• W2008686724 endingPage "025301" @default.
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• W2008686724 abstract "We present a novel measurement method based on the gravimetric principles adapted from the ASTM E542 and ISO 4787 standards for quantitative volume determination in the sub-microliter range. Such a method is particularly important for the calibration of non-contact micro dispensers as well as other microfluidic devices. The novel method is based on the linear regression analysis of continuously monitored gravimetric results and therefore is referred to as 'gravimetric regression method (GRM)'. In this context, the regression analysis is necessary to compensate the mass loss due to evaporation that is significant for very small dispensing volumes. A full assessment of the measurement uncertainty of GRM is presented and results in a standard measurement uncertainty around 6 nl for dosage volumes in the range from 40 nl to 1 µl. The GRM has been experimentally benchmarked with a dual-dye ratiometric photometric method (Artel Inc., Westbrook, ME, USA), which can provide traceability of measurement to the International System of Units (SI) through reference standards maintained by NIST. Good precision (max. CV = 2.8%) and consistency (bias around 7 nl in the volume range from 40 to 400 nl) have been observed comparing the two methods. Based on the ASTM and ISO standards on the one hand and the benchmark with the photometric method on the other hand, two different approaches for establishing traceability for the GRM are discussed." @default.
• W2008686724 created "2016-06-24" @default.
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• W2008686724 date "2012-12-13" @default.
• W2008686724 modified "2023-09-29" @default.
• W2008686724 title "Novel gravimetric measurement technique for quantitative volume calibration in the sub-microliter range" @default.
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• W2008686724 doi "https://doi.org/10.1088/0957-0233/24/2/025301" @default.
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