FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2008691467> ?p ?o ?g. }

• W2008691467 endingPage "1800" @default.
• W2008691467 startingPage "1793" @default.
• W2008691467 abstract "Tyrosine hydroxylase (TH) is expressed specifically in catecholaminergic cells. We have identified a novel regulatory sequence in the upstream region of the bovine TH gene promoter formed by a dyad symmetry element (DSE1;-352/-307 bp). DSE1 supports TH promoter activity in TH-expressing bovine adrenal medulla chromaffin (BAMC) cells and inhibits promoter activity in non-expressing TE671 cells. DNase I footprinting of relaxed TH promoter DNA showed weak binding of nuclear BAMC cell proteins to a short sequence in the right DSE1 arm. In BAMC cells, deletion of the right arm markedly reduced the expression of luciferase from the TH promoter. However, deletion of the left DSE1 arm or its reversed orientation (RevL) also inactivated the TH promoter. In supercoiled TH promoter, DSE1 assumes a cruciform-like conformation i.e., it binds cruciform-specific 2D3 antibody, and S1 nuclease-cleavage and OsO4-modification assays have identified an imperfect cruciform extruded by the DSE1. DNase I footprinting of supercoiled plasmid showed that cruciformed DSE1 is targeted by nuclear proteins more efficiently than the linear duplex isomer and that the protected site encompasses the left arm and center of DSE1. Our results suggest that the disruption of intrastrand base-pairing preventing cruciform formation and protein binding to DSE1 is responsible for its inactivation in DSE1 mutants. DSE1 cruciform may act as a target site for activator (BAMC cells) and repressor (TE671) proteins. Its extrusion emerges as a novel mechanism that controls cellspecific promoter activity." @default.
• W2008691467 created "2016-06-24" @default.
• W2008691467 creator A5011596443 @default.
• W2008691467 creator A5032753064 @default.
• W2008691467 creator A5045791329 @default.
• W2008691467 creator A5067412714 @default.
• W2008691467 creator A5069027122 @default.
• W2008691467 date "1998-04-01" @default.
• W2008691467 modified "2023-09-25" @default.
• W2008691467 title "Cruciform-extruding regulatory element controls cell-specific activity of the tyrosine hydroxylase gene promoter" @default.
• W2008691467 cites W1165430331 @default.
• W2008691467 cites W1492984283 @default.
• W2008691467 cites W1523642307 @default.
• W2008691467 cites W1555697162 @default.
• W2008691467 cites W1560846668 @default.
• W2008691467 cites W1561471115 @default.
• W2008691467 cites W1564217760 @default.
• W2008691467 cites W1582252002 @default.
• W2008691467 cites W1583309132 @default.
• W2008691467 cites W1590365839 @default.
• W2008691467 cites W16359034 @default.
• W2008691467 cites W1966563762 @default.
• W2008691467 cites W1969669997 @default.
• W2008691467 cites W1970419257 @default.
• W2008691467 cites W1971244903 @default.
• W2008691467 cites W1974325040 @default.
• W2008691467 cites W1975383049 @default.
• W2008691467 cites W1975942578 @default.
• W2008691467 cites W1976291519 @default.
• W2008691467 cites W1980659071 @default.
• W2008691467 cites W1980910932 @default.
• W2008691467 cites W1983601901 @default.
• W2008691467 cites W1988361831 @default.
• W2008691467 cites W1993243592 @default.
• W2008691467 cites W1993528388 @default.
• W2008691467 cites W1997054462 @default.
• W2008691467 cites W1999669099 @default.
• W2008691467 cites W2003943599 @default.
• W2008691467 cites W2007147088 @default.
• W2008691467 cites W2009184402 @default.
• W2008691467 cites W2013804264 @default.
• W2008691467 cites W2013844478 @default.
• W2008691467 cites W2015292413 @default.
• W2008691467 cites W2016103240 @default.
• W2008691467 cites W2025830975 @default.
• W2008691467 cites W2035468974 @default.
• W2008691467 cites W2037018067 @default.
• W2008691467 cites W2038962015 @default.
• W2008691467 cites W2046184823 @default.
• W2008691467 cites W2048757240 @default.
• W2008691467 cites W2063125022 @default.
• W2008691467 cites W2064232231 @default.
• W2008691467 cites W2064769526 @default.
• W2008691467 cites W2067926870 @default.
• W2008691467 cites W2077332064 @default.
• W2008691467 cites W2077667025 @default.
• W2008691467 cites W2078124710 @default.
• W2008691467 cites W2091775431 @default.
• W2008691467 cites W2095531021 @default.
• W2008691467 cites W2111386319 @default.
• W2008691467 cites W2169491235 @default.
• W2008691467 cites W2171791457 @default.
• W2008691467 cites W2178168667 @default.
• W2008691467 cites W2414036700 @default.
• W2008691467 cites W317733335 @default.
• W2008691467 cites W4241645684 @default.
• W2008691467 cites W967986046 @default.
• W2008691467 doi "https://doi.org/10.1093/nar/26.7.1793" @default.
• W2008691467 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/147441" @default.
• W2008691467 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9512554" @default.
• W2008691467 hasPublicationYear "1998" @default.
• W2008691467 type Work @default.
• W2008691467 sameAs 2008691467 @default.
• W2008691467 citedByCount "27" @default.
• W2008691467 countsByYear W20086914672013 @default.
• W2008691467 countsByYear W20086914672016 @default.
• W2008691467 countsByYear W20086914672017 @default.
• W2008691467 countsByYear W20086914672020 @default.
• W2008691467 crossrefType "journal-article" @default.
• W2008691467 hasAuthorship W2008691467A5011596443 @default.
• W2008691467 hasAuthorship W2008691467A5032753064 @default.
• W2008691467 hasAuthorship W2008691467A5045791329 @default.
• W2008691467 hasAuthorship W2008691467A5067412714 @default.
• W2008691467 hasAuthorship W2008691467A5069027122 @default.
• W2008691467 hasBestOaLocation W20086914671 @default.
• W2008691467 hasConcept C101762097 @default.
• W2008691467 hasConcept C104317684 @default.
• W2008691467 hasConcept C107824862 @default.
• W2008691467 hasConcept C150194340 @default.
• W2008691467 hasConcept C153911025 @default.
• W2008691467 hasConcept C158448853 @default.
• W2008691467 hasConcept C165864922 @default.
• W2008691467 hasConcept C166957645 @default.
• W2008691467 hasConcept C21592294 @default.
• W2008691467 hasConcept C2776086361 @default.
• W2008691467 hasConcept C2778021871 @default.
• W2008691467 hasConcept C2779377703 @default.
• W2008691467 hasConcept C3017666073 @default.

• next