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• W2008697930 abstract "20-HETE is a potent, vasoconstrictive arachidonic acid metabolite with a limited number of published methods for quantitative assessment of microsomal formation rate. The purpose of this study was to evaluate the utility of HPLC-MS (negative ESI) for quantitation of rat microsomal 20-HETE enzyme kinetics. Calibration curves were linear over 0.75-16 ng on-column (r(2)>0.996). The intra- and inter-assay precision and accuracy were <15%. Microsomal 20-HETE revealed saturable (100 microM) kinetics (brain K(m) and V(max): 39.9+/-6.0 microM and 8.7+/-0.6 pM/min per mg; liver K(m) and V(max): 23.5+/-3.2 microM and 775.5+/-39.8 pmol/min per mg; kidney K(m) and V(max): 47.6+/-8.5 microM and 1933+/-151 pM/min per mg). This paper demonstrates HPLC-MS as an efficient method for quantitating 20-HETE enzyme kinetics in microsomes from rat tissues." @default.
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• W2008697930 date "2003-09-01" @default.
• W2008697930 modified "2023-09-26" @default.
• W2008697930 title "Determination of 20-hydroxyeicosatetraenoic acid in microsomal incubates using high-performance liquid chromatography–mass spectrometry (HPLC–MS)" @default.
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• W2008697930 doi "https://doi.org/10.1016/s1570-0232(03)00496-3" @default.
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