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• W2008734588 abstract "Transcription promoter of the aldolase B gene was previously shown to be centered on an initiation region of DNA replication in rat hepatoma cells in vivo. Here, we defined an essential region required for replication in a plasmid form upon transfection. Deletion analyses around the origin region revealed that the proximal 200 bp promoter was necessary, but not sufficient for replication as flanking sequence restored replication activity. Therefore, the 200 bp region seemed to cooperate with the flanking sequence to play an important role in replication. Electrophoretic mobility shift assays using nuclear extracts from synchronously growing hepatoma cells showed that some protein factors bound to this region in a cell cycle-regulated manner. Since transcription of the aldolase B gene is repressed in the hepatoma cells, the cell cycle-regulated protein-binding is considered to be involved in regulation of replication initiation." @default.
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• W2008734588 date "1997-08-01" @default.
• W2008734588 modified "2023-09-26" @default.
• W2008734588 title "Sequence Requirement for Replication Initiation at the Rat Aldolase B Locus Implicated in Its Functional Correlation with Transcriptional Regulation" @default.
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• W2008734588 doi "https://doi.org/10.1006/bbrc.1997.7216" @default.
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